Przewłocka B, Turchan J, Lasoń W, Przewłocki R
Department of Molecular Neuropharmacology, Institute of Pharmacology, Polish Academy of Sciences, Krakow.
Neurosci Lett. 1997 Nov 28;238(1-2):13-6. doi: 10.1016/s0304-3940(97)00829-x.
The present study investigated the effects of ethanol withdrawal after its chronic administration on endogenous opioid systems in the nucleus accumbens of rats. An in situ hybridization study showed an increase in the prodynorphin mRNA level at 24 and 48 h (by 189 and 146%, respectively) after ethanol withdrawal, whereas the proenkephalin mRNA level remained unchanged. Furthermore, after a 48 h withdrawal period, the level of alpha-neoendorphin (alphaNEO), a prodynorphin-derived peptide, was significantly decreased (by 48%), that effect being associated with the enhancement of the K+-stimulated release of that peptide from nucleus accumbens slices. At 96 h after ethanol withdrawal, only the basal release of alphaNEO was elevated, while other parameters returned to the control level. Our data indicate that after 48 h of ethanol withdrawal, prodynorphin neurons are highly activated. The increased supply of endogenous kappa opioid receptor agonists in the nucleus accumbens at that time may promote aversive states during ethanol withdrawal.
本研究调查了长期给予乙醇后戒断对大鼠伏隔核内源性阿片系统的影响。原位杂交研究显示，乙醇戒断后24小时和48小时，前强啡肽原mRNA水平升高（分别升高189%和146%），而前脑啡肽原mRNA水平保持不变。此外，在戒断48小时后，强啡肽原衍生肽α-新内啡肽（αNEO）的水平显著降低（降低48%），该效应与伏隔核切片中该肽的钾离子刺激释放增强有关。乙醇戒断96小时后，仅αNEO的基础释放升高，而其他参数恢复到对照水平。我们的数据表明，乙醇戒断48小时后，前强啡肽原神经元被高度激活。此时伏隔核内源性κ阿片受体激动剂供应增加可能会在乙醇戒断期间促进厌恶状态。
