Golsteyn R M, Louvard D, Friederich E
Morphogenèse et Signalisation Cellulaires, Centre National de la Recherche Scientifique, UMR 144, Institut Curie, Paris, France.
Biophys Chem. 1997 Oct;68(1-3):73-82. doi: 10.1016/s0301-4622(97)00009-4.
We summarize recent findings on the organization of the protein actin in eucaryotic cells. In particular we focus on how actin can be used to generate a vectorial force that is required for cell movement. These forces arise from protein molecules that recruit actin to the plasma membrane in such a manner that actin filaments extend outward from the cell body. This type of actin dependent force generation has been described in a nucleation-release model, which is one of several models currently being tested to explain actin dependent cell movement. Data in support of this model has arisen unexpectedly from studies of an intracellular bacteria, Listeria monocytogenes. This bacteria uses actin to propel itself during infection of eucaryotic cells. By studying Listeria movement, the roles of several eucaryotic actin interacting proteins have been identified. One of these is zyxin, a human protein that shares important structural and possibly functional properties with ActA, an actin dependent force generating protein of Listeria. We intend to test the function of these and other actin interacting proteins in a simplified system that should facilitate precise measurement of their properties of force generation in vitro.
我们总结了真核细胞中肌动蛋白组织的最新研究结果。特别地，我们关注肌动蛋白如何用于产生细胞运动所需的矢量力。这些力源自将肌动蛋白募集到质膜的蛋白质分子，使得肌动蛋白丝从细胞体向外延伸。这种依赖肌动蛋白的力的产生已在成核 - 释放模型中得到描述，该模型是目前为解释依赖肌动蛋白的细胞运动而正在测试的几种模型之一。支持该模型的数据意外地来自对细胞内细菌单核细胞增生李斯特菌的研究。这种细菌在感染真核细胞期间利用肌动蛋白推动自身。通过研究李斯特菌的运动，已鉴定出几种真核肌动蛋白相互作用蛋白的作用。其中之一是桩蛋白，一种人类蛋白，它与李斯特菌的肌动蛋白依赖性力产生蛋白ActA具有重要的结构和可能的功能特性。我们打算在一个简化系统中测试这些以及其他肌动蛋白相互作用蛋白的功能，该系统应有助于在体外精确测量它们产生力的特性。
