The role of ganglioside GM3 in the modulation of conformation and activity of sarcoplasmic reticulum Ca(2+)-ATPase.
作者信息
Yang F Y, Wang L H, Yang X Y, Tsui Z C, Tu Y P
机构信息
National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
出版信息
Biophys Chem. 1997 Oct;68(1-3):137-46. doi: 10.1016/s0301-4622(97)00025-2.
PMID:9468616
Abstract
Rabbit sarcoplasmic reticulum does contain trace amounts of gangliosides, and the main species is GM3. Incorporation of GM3 into the SR vesicles or addition of it to the soybean phospholipid used for reconstitution of proteoliposomes obviously increased ATP hydrolysis, as well as, Ca2+ uptake activity of sarcoplasmic reticulum Ca(2+)-ATPase. Conformation changes of Ca(2+)-ATPase induced by GM3 were also observed by circular dichroism, intrinsic fluorescence and fluorescence quenching measurements.
摘要
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