Namba R S, Meuli M, Sullivan K M, Le A X, Adzick N S
Department of Orthopaedic Surgery, University of California at San Francisco, 94143-0728, USA.
J Bone Joint Surg Am. 1998 Jan;80(1):4-10. doi: 10.2106/00004623-199801000-00003.
A fetal lamb model was developed to investigate the capacity of fetal articular cartilage for repair after the creation of a superficial defect. Superficial defects, 100 micrometers deep, were made in the articular cartilage of the trochlear groove in the distal aspect of the femur in eighteen fetal lambs that were halfway through the 145-day gestational period; the contralateral limb was used as a sham control. The wounds were allowed to heal in utero for three, seven, fourteen, twenty-one, or twenty-eight days. Seven days after the injury, the defects were filled with a hypocellular matrix, which stained lightly with safranin O. At twenty-eight days, the staining of the matrix was similar to that of the sham controls and the chondrocyte density and the architectural arrangement of the cell layers had been restored. An inflammatory response was not elicited, and no fibrous scar tissue was observed.
建立了一个胎羊模型，以研究在制造浅表缺损后胎儿关节软骨的修复能力。在18只处于145天妊娠期中期的胎羊的股骨远端滑车沟关节软骨中制造了深度为100微米的浅表缺损；对侧肢体用作假手术对照。伤口在子宫内愈合3、7、14、21或28天。损伤后7天，缺损处充满了细胞含量少的基质，用番红O染色较浅。在28天时，基质的染色与假手术对照相似，软骨细胞密度和细胞层的结构排列已恢复。未引发炎症反应，也未观察到纤维瘢痕组织。
