Neuner P, Cortese R, Monaci P
Chemistry Department, Istituto di Ricerche di Biologia Molecolare P. Angeletti, Via Pontina km 30.600, 00040 Pomezia (Roma), Italy.
Nucleic Acids Res. 1998 Mar 1;26(5):1223-7. doi: 10.1093/nar/26.5.1223.
A new approach for the synthesis of randomized DNA sequences containing the 20 codons corresponding to all natural amino acids is described. The strategy is based on the use of dinucleotide phosphoramidite building blocks within a resin-splitting procedure. Through this protocol, a minimal number of seven dimers is sufficient to encode all 20 natural amino acids. This synthesis procedure is extremely flexible and allows codon usage from different hosts to be accommodated.
本文描述了一种合成包含对应于所有天然氨基酸的20个密码子的随机DNA序列的新方法。该策略基于在树脂拆分程序中使用二核苷酸亚磷酰胺构建块。通过该方案,最少七个二聚体就足以编码所有20种天然氨基酸。这种合成程序极其灵活,能够适应不同宿主的密码子使用情况。