在通透完整细胞中对天然基因进行三链体靶向：趋化因子受体CCR5基因的共价修饰
Triplex targeting of a native gene in permeabilized intact cells: covalent modification of the gene for the chemokine receptor CCR5.
作者信息
Belousov E S, Afonina I A, Kutyavin I V, Gall A A, Reed M W, Gamper H B, Wydro R M, Meyer R B
机构信息
Epoch Pharmaceuticals Inc., 1725 220th Street S.E., #104 Bothell, WA 98021, USA.
出版信息
Nucleic Acids Res. 1998 Mar 1;26(5):1324-8. doi: 10.1093/nar/26.5.1324.
Abstract
A 12 nucleotide oligodeoxyribopurine tract in the gene for the chemokine receptor CCR5 has been targeted and covalently modified in intact cells by a 12mer triplex forming oligonucleotide (TFO) bearing a reactive group. A nitrogen mustard placed on the 5'-end of the purine motif TFO modified a guanine on the DNA target with high efficiency and selectivity. A new use of a guanine analog in these TFOs significantly enhanced triplex formation and efficiency of modification, as did the use of the triplex-stabilizing intercalator coralyne. This site-directed modification of a native chromosomal gene in intact human cells under conditions where many limitations of triplex formation have been partially addressed underscores the potential of this approach for gene control via site-directed mutagenesis.
摘要
趋化因子受体CCR5基因中的一段12个核苷酸的寡聚脱氧嘌呤序列已被带有反应基团的12聚体三链形成寡核苷酸（TFO）靶向，并在完整细胞中进行了共价修饰。置于嘌呤基序TFO 5'端的氮芥高效且选择性地修饰了DNA靶标上的一个鸟嘌呤。在这些TFO中使用鸟嘌呤类似物显著增强了三链形成和修饰效率，三链稳定嵌入剂珊瑚霉素的使用也有同样效果。在部分解决了三链形成诸多限制的条件下，对完整人类细胞中的天然染色体基因进行这种位点定向修饰，突出了该方法通过位点定向诱变进行基因控制的潜力。
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