Ichikawa T, Shimizu T
Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Japan.
Brain Res. 1998 Jan 1;779(1-2):96-103. doi: 10.1016/s0006-8993(97)01090-1.
Cholinergic structures in the cranial nerve motor nuclei and ventral and lateral horns of the spinal cord of the monkey, Macaca fuscata, were investigated immunohistochemically with a monoclonal antibody against monkey choline acetyltransferase (ChAT). ChAT-immunoreactive perikarya and dendrites were present in the oculomotor, trochlear, abducent, trigeminal motor, facial and hypoglossal nuclei, nucleus of Edinger-Westphal, nucleus ambiguus, dorsal nucleus of the vagus, lamina IX of the cervical, thoracic and lumbar spinal cords, and intermediolateral nucleus of the thoracic spinal cord. The neuropil of the trigeminal motor, facial and hypoglossal nuclei, nucleus ambiguus and lamina IX of the cervical, thoracic and lumbar spinal cords contained many ChAT-positive bouton-like structures and they were seemingly in contact with perikarya and dendrites of motoneurons, suggesting that motoneurons in these nuclei are cholinoceptive as well as cholinergic. The oculomotor, trochlear and abducent nuclei, nucleus of Edinger-Westphal, dorsal nucleus of the vagus and intermediolateral nucleus of the thoracic spinal cord contained a small number of ChAT-immunoreactive bouton-like structures, but they did not contact with perikarya and dendrites of ChAT-positive neurons. These observations suggest that the organization of the motor nuclei is complex, at least regarding the cholinoceptivity.
使用抗猴胆碱乙酰转移酶（ChAT）的单克隆抗体，通过免疫组织化学方法对猕猴（Macaca fuscata）的脑神经运动核以及脊髓腹角和外侧角中的胆碱能结构进行了研究。ChAT免疫反应阳性的胞体和树突存在于动眼神经核、滑车神经核、展神经核、三叉神经运动核、面神经核和舌下神经核、动眼神经副核、疑核、迷走神经背核、颈、胸和腰脊髓的第IX层以及胸脊髓的中间外侧核中。三叉神经运动核、面神经核和舌下神经核、疑核以及颈、胸和腰脊髓第IX层的神经毡含有许多ChAT阳性的纽扣样结构，它们似乎与运动神经元的胞体和树突相接触，这表明这些核中的运动神经元既是胆碱能的也是胆碱感受性的。动眼神经核、滑车神经核和展神经核、动眼神经副核、迷走神经背核以及胸脊髓的中间外侧核含有少量ChAT免疫反应阳性的纽扣样结构，但它们不与ChAT阳性神经元的胞体和树突接触。这些观察结果表明，至少就胆碱感受性而言，运动核的组织结构是复杂的。
