大鼠D-氨基酸氧化酶cDNA：大鼠D-氨基酸氧化酶作为小鼠和其他哺乳动物D-氨基酸氧化酶之间的一种中间形式。

Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases.

作者信息

Konno R

机构信息

Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi, Japan.

出版信息

Biochim Biophys Acta. 1998 Jan 21;1395(2):165-70. doi: 10.1016/s0167-4781(97)00185-1.

DOI:10.1016/s0167-4781(97)00185-1

PMID:9473656

Abstract

Nucleotide sequence of cDNA encoding rat D-amino-acid oxidase (DAO) was determined. Two species of DAO mRNA were present in rat kidney, liver, and brain. They were probably produced by alternative splicing. Rat DAO cDNA encodes 346 amino acid residues, indicating that rat DAO is an intermediate form between mouse DAO (345 amino acids) and DAOs (347 amino acids) of human, rabbit, and pig. Deduced amino acid sequence indicates 93% identity between rat and mouse DAO. Northern hybridization and western blotting supported the sequence data.

摘要

测定了编码大鼠D - 氨基酸氧化酶（DAO）的cDNA的核苷酸序列。大鼠肾脏、肝脏和大脑中存在两种DAO mRNA。它们可能是通过可变剪接产生的。大鼠DAO cDNA编码346个氨基酸残基，这表明大鼠DAO是小鼠DAO（345个氨基酸）与人类、兔子和猪的DAO（347个氨基酸）之间的中间形式。推导的氨基酸序列表明大鼠和小鼠DAO之间有93%的同一性。Northern杂交和蛋白质免疫印迹法支持了序列数据。