Gonschior P, Vogel-Wiens C, Goetz A E, Huehns T Y, Breger F, Gerheuser F, Fleuchaus M, Welsch U, Sroka R, Dellian M, Lehr H A, Höfling B
Medical Department, Klinikum Grosshadern, Munich, Germany.
Basic Res Cardiol. 1997 Oct;92(5):310-9. doi: 10.1007/BF00788943.
The effectiveness of local endovascular photodynamic therapy (PDT) in preventing tissue hyperplasia was evaluated in a vascular injury model.
Standardized unidirectional arterial injury with a directional atherectomy catheter was performed in porcine arteries (n = 180). Animals (n = 72) were randomly allocated to unidirectional injury only (Group 1), injury followed by drug delivery of photosensitizer with a porous balloon (Group 2), or by local exposure to monochromatic light (Group 3). In Group 4, injury was followed by local drug delivery of photosensitizer and subsequent exposure to light (PDT). Up to 21 days after treatment, all experimental vessels were excised, fixed and processed for histology, immunohistochemistry and transmission electron microscopy.
After vascular injury an inflammatory and myoproliferative response was observed in Groups 1, 2 and 3 (mean tissue hyperplasia/media ratio 1.0 +/- 0.5 at 21 days; area tissue hyperplasia: 1.57 +/- 0.9 mm2). Proliferation in injured vascular segments (Group 1-3) reached a maximum at 7 days, with 6%. Only in Group 4, after injury followed by photodynamic therapy, was there no significant vascular response (mean tissue hyperplasia/media ratio 0.3 +/- 0.2: area tissue hyperplasia: 0.1 +/- 0.05 mm2 p < 0.001, proliferating cells 0.3%).
Vascular response after unidirectional injury was suppressed only by endovascular photodynamic therapy.
在血管损伤模型中评估局部血管内光动力疗法（PDT）预防组织增生的有效性。
使用定向旋切导管在猪动脉（n = 180）中进行标准化单向动脉损伤。将动物（n = 72）随机分配至仅单向损伤组（第1组）、损伤后用多孔球囊递送光敏剂组（第2组）或局部暴露于单色光组（第3组）。在第4组中，损伤后进行局部光敏剂给药并随后暴露于光（PDT）。治疗后长达21天，切除所有实验血管，固定并进行组织学、免疫组织化学和透射电子显微镜检查。
在第1、2和3组中观察到血管损伤后的炎症和肌增生反应（21天时平均组织增生/中膜比为1.0 +/- 0.5；组织增生面积：1.57 +/- 0.9 mm2）。损伤血管段（第1 - 3组）的增殖在第7天达到最大值，为6%。仅在第4组中，损伤后进行光动力治疗，未观察到明显的血管反应（平均组织增生/中膜比为0.3 +/- 0.2：组织增生面积：0.1 +/- 0.05 mm2，p < 0.001，增殖细胞为0.3%）。
仅血管内光动力疗法可抑制单向损伤后的血管反应。
