Ha H C, Yager J D, Woster P A, Casero R A
Department of Environmental Health Sciences, Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205, USA.
Biochem Biophys Res Commun. 1998 Mar 6;244(1):298-303. doi: 10.1006/bbrc.1998.8258.
Reactive oxygen species are known to induce strand breaks and/or base modifications in DNA. DNA strand breaks are associated with many pathologies and programmed cell death. We have examined the ability of the polyamines and their analogues to protect phi X-174 plasmid DNA from strand breakage induced by a oxygen-radical generating system. Spermine and several unsymmetrically substituted polyamine analogues reduced the amount of strand breakage at a physiologically relevant concentration of 1 mM. However, putrescine, spermidine, N1-acetylspermine, N1-acetylspermidine and symmetrically alkylated polyamine analogues were not able to reduce strand breakage at the same concentration. Thus, the unsymmetrically alkylated polyamine analogues and natural spermine can protect DNA against strand breakage induced by Cu(II)/H2O2 generated ROS similar to other more classical antioxidants.
已知活性氧会诱导DNA链断裂和/或碱基修饰。DNA链断裂与许多病理状态和程序性细胞死亡有关。我们研究了多胺及其类似物保护phi X-174质粒DNA免受氧自由基生成系统诱导的链断裂的能力。在1 mM的生理相关浓度下，精胺和几种不对称取代的多胺类似物减少了链断裂的量。然而，腐胺、亚精胺、N1-乙酰精胺、N1-乙酰亚精胺和对称烷基化的多胺类似物在相同浓度下不能减少链断裂。因此，不对称烷基化的多胺类似物和天然精胺可以保护DNA免受Cu(II)/H2O2产生的ROS诱导的链断裂，类似于其他更经典的抗氧化剂。
