Santos J, Saperas E, Nogueiras C, Mourelle M, Antolín M, Cadahia A, Malagelada J R
Digestive System Research Unit, Hospital General Vall d'Hebron, Autonomous University of Barcelona, Barcelona, Spain.
Gastroenterology. 1998 Apr;114(4):640-8. doi: 10.1016/s0016-5085(98)70577-3.
BACKGROUND & AIMS: The central nervous system regulates gut functions via complex interactions between the enteric nervous and immune systems. The aim of this study was to investigate whether mast cell mediators are released into the human jejunal lumen during stress.
A closed-segment perfusion technique was used to investigate jejunal release of tryptase, histamine, prostaglandin D2, and water flux in response to the cold pressor test in 8 healthy subjects and 9 patients with food allergy. In 6 food-allergic patients, jejunal biochemical responses to cold pain stress were compared with those induced by food intraluminal challenge.
Cold pain stress elevated heart rate and blood pressure and increased luminal release of mast cell mediators and jejunal water secretion in both groups. Stress-induced release of tryptase and histamine, but not of prostaglandin D2 and water flux, was greater in food-allergic patients than in healthy volunteers. In food-allergic patients, jejunal biochemical responses induced by cold pain stress were similar to those induced by antigen challenge.
These results show the ability of the central nervous system to modulate intestinal mast cell activity and suggest that mast cells have a role in stress-related gut dysfunction.
中枢神经系统通过肠神经系统与免疫系统之间的复杂相互作用来调节肠道功能。本研究旨在调查应激期间肥大细胞介质是否释放到人类空肠腔中。
采用封闭段灌注技术，研究8名健康受试者和9名食物过敏患者在冷加压试验后空肠中类胰蛋白酶、组胺、前列腺素D2的释放及水通量。在6名食物过敏患者中，比较了空肠对冷痛应激的生化反应与腔内食物激发试验所诱导的反应。
冷痛应激使两组受试者的心率和血压升高，并增加了肥大细胞介质的腔内释放及空肠水分泌。食物过敏患者应激诱导的类胰蛋白酶和组胺释放，但前列腺素D2和水通量释放，比健康志愿者更大。在食物过敏患者中，冷痛应激诱导的空肠生化反应与抗原激发试验诱导的反应相似。
这些结果表明中枢神经系统能够调节肠道肥大细胞活性，并提示肥大细胞在应激相关的肠道功能障碍中起作用。
