Matsukura Y, Takagi T, Okamoto R, Koshino T
Department of Orthopaedic Surgery, School of Medicine, Yokohama City University, Yokohama, Japan.
J Rheumatol. 1998 Mar;25(3):539-45.
To investigate aspects of the inflammatory process of the mouse subcutaneous air pouch -- a facsimile synovial cavity -- induced by injection of lipid A, and to determine the expression and upregulation of CD44 in the lining cell layer of the inflamed air pouch.
Histological changes of inner walls in the mouse air pouch were evaluated 1, 3, and 7 days after injection of lipid A.
Polymorphonuclear cell infiltration in the lining layer reached the maximum one day after injection of 10 microg of lipid A (10/10 mice in Grade 3; p < 0.01), while mononuclear cell infiltration and lining cell hyperplasia reached the maximum 3 days after injection (5/10 mice in Grade 2; 5/10 in Grade 3; p < 0.05; 39+/-11 layers, p < 0.05, respectively). The number of cell depth of CD44 positive lining layers and interleukin 1alpha (IL-1alpha) positive lining layers reached the maximum 3 days after injection (39+/-7 layers, p < 0.01; 35+/-12 layers, p < 0.05, respectively).
These findings suggest that CD44 may have some connection with the proinflammatory cytokine IL-1alpha and induce inflammatory responses in the air pouch injected with lipid A.
研究注射脂多糖诱导的小鼠皮下气囊——一种模拟滑膜腔——炎症过程的相关方面，并确定炎症气囊内衬细胞层中CD44的表达及上调情况。
在注射脂多糖后1天、3天和7天评估小鼠气囊内壁的组织学变化。
注射10微克脂多糖后1天，内衬层多形核细胞浸润达到最大值（10只小鼠中有10只达到3级；p<0.01），而单核细胞浸润和内衬细胞增生在注射后3天达到最大值（10只小鼠中有5只达到2级；5只达到3级；p<0.05；分别为39±11层，p<0.05）。CD44阳性内衬层和白细胞介素1α（IL-1α）阳性内衬层的细胞深度在注射后3天达到最大值（分别为39±7层，p<0.01；35±12层，p<0.05）。
这些发现表明，CD44可能与促炎细胞因子IL-1α存在某种关联，并在注射脂多糖的气囊中诱导炎症反应。
