Hardy J, Bertone A L, Malemud C J
Department of Veterinary Clinical Sciences, Ohio State University, Columbus 43210, USA.
Am J Vet Res. 1998 Mar;59(3):293-9.
To determine the functional response of synovium to infection, and the influence of infected synovium on articular cartilage metabolism.
Synovium and articular cartilage explants from the midcarpal and tarsocrural joints of adult horses.
For experiment 1, synovium explants were incubated as follows: control--incubation in standard medium, infected (I)--incubation with Staphylococcus aureus, and infected-filtered (IF)--incubation with medium collected from the infected group and filtered (0.22-micron filter). Daily collected medium was assayed for interleukin 1 beta (IL-1 beta), IL-6, tumor necrosis factor, and hyaluronan (HA) concentrations. For experiment 2, cartilage explants were incubated as follows: control--incubation in standard medium, and IF--incubation in medium collected from infected synovium cultures and filtered. After 48 hours, explant proteoglycan synthesis and endogenous proteoglycan and glycosaminoglycan contents were determined.
IL-1 beta and IL-6 values were significantly increased in synovium explants from the I and IF groups. Hyaluronan concentration was lower in I and IF groups. Proteoglycan synthesis and content, and total glycosaminoglycan and chondroitin sulfate concentrations, were significantly decreased in cartilage from the IF group.
Bacterial infection was associated with decreased HA concentration and increased mediator release. These effects were also observed despite elimination of bacteria. Exposure to sterile but previously infected medium decreased articular cartilage matrix synthesis and composition.
Resident synovial cells may contribute appreciably to articular damage during bacterial infection in the absence of migrant inflammatory cells. This response is prolonged despite elimination of the bacteria.
确定滑膜对感染的功能反应，以及感染的滑膜对关节软骨代谢的影响。
成年马腕中关节和跗关节的滑膜及关节软骨外植体。
实验1中，滑膜外植体按以下方式培养：对照组——在标准培养基中培养；感染组（I）——与金黄色葡萄球菌共同培养；感染过滤组（IF）——与从感染组收集并过滤（0.22微米滤器）的培养基共同培养。每天收集的培养基用于检测白细胞介素1β（IL-1β）、IL-6、肿瘤坏死因子和透明质酸（HA）浓度。实验2中，软骨外植体按以下方式培养：对照组——在标准培养基中培养；IF组——在从感染的滑膜培养物中收集并过滤的培养基中培养。48小时后，测定外植体蛋白聚糖合成以及内源性蛋白聚糖和糖胺聚糖含量。
I组和IF组滑膜外植体中IL-1β和IL-6值显著升高。I组和IF组透明质酸浓度较低。IF组软骨中蛋白聚糖合成及含量、总糖胺聚糖和硫酸软骨素浓度显著降低。
细菌感染与HA浓度降低和介质释放增加有关。即使消除细菌，这些影响仍然存在。暴露于无菌但先前感染过的培养基会降低关节软骨基质的合成和组成。
在没有迁移性炎症细胞的情况下，常驻滑膜细胞在细菌感染期间可能对关节损伤有显著影响。即使消除细菌，这种反应仍会持续。
