May C D
Ann Allergy. 1976 Jul;37(1):8-11.
Because histamine occurs naturally in some food products, quantitative analysis of the histamine content of extracts of food used for skin testing seemed desirable to determine its effect in the production of positive reactions. Up to 200 nanograms of histamine per milliliter were found in some food extracts. When the extracts are diluted to 1:100 and 1:1000 W/V for skin testing the amount of histamine which would be injected in a intradermal skin test is one-thousandth of the amount required to produce a significant wheal. Therefore the histamine content of the foods analyzed is too small to be of practical concern and not enough to give nonspecific wheal reactions in intradermal tests using extracts of 1/1000 or 1/100 W/V concentrations. Nonspecific reactions in skin tests are probably most often due to use of food extracts of unnecessarily high concentration.
由于组胺天然存在于某些食品中，因此对用于皮肤测试的食品提取物中的组胺含量进行定量分析，似乎有助于确定其在产生阳性反应中的作用。在一些食品提取物中发现每毫升高达200纳克的组胺。当将提取物以重量/体积比稀释至1:100和1:1000用于皮肤测试时，皮内皮肤测试中注射的组胺量是产生明显风团所需量的千分之一。因此，所分析食品中的组胺含量太小，无需实际关注，并且不足以在使用1/1000或1/100重量/体积浓度提取物的皮内测试中产生非特异性风团反应。皮肤测试中的非特异性反应可能最常归因于使用了不必要高浓度的食品提取物。
