Venkatraj V S, Begemann M, Sobrino A, Bruce J N, Weinstein I B, Warburton D
Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
J Neurooncol. 1998 Jan;36(2):141-8. doi: 10.1023/a:1005859318971.
Comparative genomic hybridization serves as a screening test for regions of copy number changes in tumor genomes. We have applied the technique to map DNA gains and losses in 5 cell lines derived from glioblastoma multiforme, the most common primary neoplasm of the central nervous system. The most frequent losses occurred on chromosomes 10 and 13. The most common gains were observed on chromosomes 5, 6, 7 and 20. Some novel sites of genomic alterations were also observed. Analysis of common areas of loss and gain in these cell lines provides a basis for future attempts to more finely map these genetic changes and for elucidation of genes involved in tumor progression.
比较基因组杂交可作为一种筛选试验，用于检测肿瘤基因组中拷贝数变化的区域。我们已应用该技术来绘制源自多形性胶质母细胞瘤（中枢神经系统最常见的原发性肿瘤）的5种细胞系中的DNA增减情况。最常见的缺失发生在10号和13号染色体上。最常见的增益出现在5号、6号、7号和20号染色体上。还观察到一些新的基因组改变位点。对这些细胞系中增减的共同区域进行分析，为未来更精确地绘制这些基因变化以及阐明参与肿瘤进展的基因提供了基础。
