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[DNA理化参数在长期储存过程中的稳定性]

[Stability of physico-chemical parameters of DNA during long-term storage].

作者信息

Kolobov A V, Vaĭnberg Iu P

出版信息

Biull Eksp Biol Med. 1976 Mar;81(3):309-12.

PMID:953276
Abstract

DNA was extracted from calf thymus and sturgeon milt and purified of admixtures; this highly polymeric DNA was studied under conditions of its prolonged storage (for 1 1/2-2 years) at a temperature of 0-4 degrees C in 1 M NaCl in the form of a precipitate under a 70% ethanol and in the lyophilized state. It appeared that DNA obtained from the mentioned sources could be stored for at least 1 1/2 years under the said conditions without any significant damage of its polymeric properties or secondary structure. Although DNA from sturgeon milt had the initial physico-chemical indices similar to those of the thymus DNA, the former displayed much greater stability under the same storage condition, indicating stability of its molecular structure.

摘要

从牛胸腺和鲟鱼精液中提取DNA并去除杂质;对这种高度聚合的DNA在0-4摄氏度下于1M氯化钠中,以70%乙醇下的沉淀形式以及冻干状态进行长达1年半至2年的长期储存条件下进行研究。结果表明,从上述来源获得的DNA在所述条件下可储存至少1年半,而其聚合特性或二级结构没有任何显著损伤。虽然鲟鱼精液中的DNA初始理化指标与胸腺DNA相似,但在相同储存条件下前者表现出更大的稳定性,表明其分子结构的稳定性。

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