Ermert L, Ermert M, Goppelt-Struebe M, Walmrath D, Grimminger F, Steudel W, Ghofrani H A, Homberger C, Duncker H, Seeger W
Department of Pathology, Justus-Liebig-University Giessen, Giessen, Germany.
Am J Respir Cell Mol Biol. 1998 Apr;18(4):479-88. doi: 10.1165/ajrcmb.18.4.2939.
Prostanoid generation may proceed via both isoforms of cyclooxygenase, Cox-1 and Cox-2. Cox-1 is thought to be ubiquitously expressed, whereas Cox-2 is mostly assumed to be dynamically regulated, responding to inflammatory stimuli. The cellular localization of Cox-1 and Cox-2 in the lung, an organ with high cyclooxygenase activity, is not known. In normal rat lungs the expression and localization of Cox-1 and Cox-2 were examined with immunogold-silver staining and the RT-PCR technique. Quantitative image analysis of the staining intensity was performed by measuring mean gray values of digitized epipolarization images. Expression of both Cox-1 and Cox-2 was readily detectable in rat lungs. Cox-1 immunoreactivity localized predominantly to bronchial epithelial cells, smooth muscle cells of large hilum veins, and (with lower expression) to alveolar macrophages and pulmonary artery endothelial cells. The most intense Cox-2 staining was noted in macrophage- and mast cell-like cells, detected in close vicinity to the bronchial epithelium and in the connective tissue surrounding the vessels. In addition, strong Cox-2 expression was found in smooth muscle cells of partially muscular vessels and large veins of the hilum. Bronchial epithelial cells displayed Cox-2 immunoreactivity with limited intensity. Alveolar macrophages and alveolar septal cells were only occasionally stained with anti-Cox-2 antibodies. Both Cox-1 and Cox-2 are constitutively expressed in several cell types of normal rat lung, but display clearly different patterns of cellular localization. Cox-2 may not be related only to lung inflammation, but is suggested to be implicated in regulatory processes under physiological conditions as well.
前列腺素的生成可通过环氧化酶的两种同工型，即Cox-1和Cox-2进行。Cox-1被认为在全身广泛表达，而Cox-2大多被假定受到动态调节，对炎症刺激作出反应。Cox-1和Cox-2在肺（一种环氧化酶活性较高的器官）中的细胞定位尚不清楚。采用免疫金银染色和RT-PCR技术，对正常大鼠肺中Cox-1和Cox-2的表达及定位进行了研究。通过测量数字化极线偏振图像的平均灰度值，对染色强度进行定量图像分析。在大鼠肺中很容易检测到Cox-1和Cox-2的表达。Cox-1免疫反应主要定位于支气管上皮细胞、大肺门静脉的平滑肌细胞，以及（表达较低）肺泡巨噬细胞和肺动脉内皮细胞。在靠近支气管上皮和血管周围结缔组织中检测到的巨噬细胞样和肥大细胞样细胞中，Cox-2染色最为强烈。此外，在部分肌性血管和平滑肌大静脉中发现了较强的Cox-2表达。支气管上皮细胞显示出强度有限的Cox-2免疫反应。肺泡巨噬细胞和肺泡间隔细胞仅偶尔被抗Cox-2抗体染色。Cox-1和Cox-2在正常大鼠肺的几种细胞类型中均有组成性表达，但细胞定位模式明显不同。Cox-2可能不仅与肺部炎症有关，还被认为在生理条件下的调节过程中也有涉及。
