神经纤维瘤病2型（NF2）相关肿瘤中merlin-p85蛋白复合物的缺失。

Loss of merlin-p85 protein complex in NF2-related tumors.

作者信息

Takeshima H, Nishi T, Yamamoto K, Kino T, Nakamura H, Saya H, Kochi M, Kuratsu J I, Ushio Y

机构信息

Department of Neurosurgery, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto, 860, Japan.

出版信息

Int J Oncol. 1998 May;12(5):1073-8. doi: 10.3892/ijo.12.5.1073.

DOI:10.3892/ijo.12.5.1073

PMID:9538131

Abstract

The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.

摘要

神经纤维瘤病2型（NF2）肿瘤抑制基因产物，即默林蛋白，属于将膜蛋白与细胞骨架相连的分子家族。我们之前已经表明，在培养细胞中，默林蛋白能与一种细胞蛋白p85进行共免疫沉淀。为了分析手术标本中默林蛋白及相关蛋白的改变情况，我们开发了一种对全细胞蛋白进行生物素标记的新方法。使用我们的方法对肿瘤组织进行筛查发现，恶性胶质瘤中没有一个以及一半的NF2相关肿瘤存在p85和默林蛋白的改变。我们的检测方法似乎对筛查NF2相关肿瘤中的默林蛋白改变很有用。