Sgorbati B, Scardovi V
Antonie Van Leeuwenhoek. 1979;45(1):129-40. doi: 10.1007/BF00400786.
Antisera were prepared against electrophoretically homogeneous transaldolase (dihydroxyacetone transferase, E.C. 2.2.1.2.) of Bifidobacterium thermophilum (B. ruminale) RU326 (ATCC25866), B. cuniculi RA93 (ATCC27916) and B. 'minimum' (homology group) F392 (ATCC 27538). Crude extracts of eighty six strains previously assigned to twenty one species of the genus Bifidobacterium on the basis of deoxyribonouclelic acid (DNA) homology (DNA-DNA hybridization), were compared by double diffusion tests on Ouchterlony plates. Eight groups of identical antigenic specificity were recognized. By analysis of the spur formation, the groups of identical specificity were arranged in preliminary sequences of decreasing similarity to each of the three homologous transaldolases used as reference points. The relationships between immunological data and the genetic similarity among the species of the genus measured by means of DNA-DNA hybridization were discussed together with some relevant points of bifidal ecology.
制备了抗嗜热双歧杆菌（反刍双歧杆菌）RU326（ATCC25866）、兔双歧杆菌RA93（ATCC27916）和“极小双歧杆菌”（同源组）F392（ATCC 27538）的电泳纯转醛醇酶（二羟基丙酮转移酶，E.C. 2.2.1.2.）的抗血清。通过在Ouchterlony平板上进行双向扩散试验，比较了先前根据脱氧核糖核酸（DNA）同源性（DNA-DNA杂交）归为双歧杆菌属21个种的86株菌株的粗提取物。识别出八组具有相同抗原特异性的菌株。通过对刺形成的分析，将具有相同特异性的组按照与用作参考点的三种同源转醛醇酶中每一种的相似性降低的初步顺序排列。讨论了免疫数据与通过DNA-DNA杂交测量的该属物种之间的遗传相似性之间的关系，以及双歧杆菌生态学的一些相关要点。
