黑色素瘤相关抗原Xp-2：从使用系统性红斑狼疮血清构建的表达文库中分离出的黑色素瘤相关抗原基因家族成员。

MAGE Xp-2: a member of the MAGE gene family isolated from an expression library using systemic lupus erythematosus sera.

作者信息

McCurdy D K, Tai L Q, Nguyen J, Wang Z, Yang H M, Udar N, Naiem F, Concannon P, Gatti R A

机构信息

Department of Pediatric Rheumatology, Children's Hospital of Orange County, California 92868, USA.

出版信息

Mol Genet Metab. 1998 Jan;63(1):3-13. doi: 10.1006/mgme.1997.2639.

DOI:10.1006/mgme.1997.2639

PMID:9538511

Abstract

Two regions of the genome contain members of the MAGE gene family; Xq27-qter and Xp21.3. We isolated a transcript, MAGE Xp-2, by screening a cDNA library from the human epithelial carcinoma cell line, HEp-2, using autoantibodies from patients with systemic lupus erythematosus (SLE). The open reading frame (ORF) of MAGE Xp-2 is entirely contained in exon 4, a signature feature of the MAGE gene family. While MAGE Xp-2 shares genomic homology with MAGE Xp-1, the predicted proteins are quite divergent. Specific primers were designed to reliably distinguish between MAGE Xp-1 and MAGE Xp-2 expression. MAGE Xp-2 is expressed in testis, but not in other normal tissues. It is also expressed strongly in two of seven melanoma cell lines and one of four breast carcinomas. MAGE gene expression may be important not only for tumor recognition and cancer therapy, but, because it is the apparent target of autoantibodies in SLE sera, it may also play a role in autoimmune diseases.

摘要

基因组的两个区域包含MAGE基因家族成员；Xq27 - qter和Xp21.3。我们使用系统性红斑狼疮（SLE）患者的自身抗体筛选人上皮癌细胞系HEp - 2的cDNA文库，分离出一种转录本MAGE Xp - 2。MAGE Xp - 2的开放阅读框（ORF）完全包含在外显子4中，这是MAGE基因家族的一个标志性特征。虽然MAGE Xp - 2与MAGE Xp - 1具有基因组同源性，但预测的蛋白质却有很大差异。设计了特异性引物以可靠地区分MAGE Xp - 1和MAGE Xp - 2的表达。MAGE Xp - 2在睾丸中表达，但在其他正常组织中不表达。它在七个黑色素瘤细胞系中的两个以及四个乳腺癌细胞系中的一个中也强烈表达。MAGE基因表达不仅可能对肿瘤识别和癌症治疗很重要，而且由于它是SLE血清中自身抗体的明显靶标，它也可能在自身免疫性疾病中起作用。