丙型肝炎病毒（HCV）解旋酶与Rep解旋酶结构的比较揭示了解旋酶SF1和SF2超家族之间的结构相似性。
Comparisons between the structures of HCV and Rep helicases reveal structural similarities between SF1 and SF2 super-families of helicases.
作者信息
Korolev S, Yao N, Lohman T M, Weber P C, Waksman G
机构信息
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
出版信息
Protein Sci. 1998 Mar;7(3):605-10. doi: 10.1002/pro.5560070309.
Abstract
Three helicase structures have been determined recently: that of the DNA helicase PcrA, that of the hepatitis C virus RNA helicase, and that of the Escherichia coli DNA helicase Rep. PcrA and Rep belong to the same super-family of helicases (SF1) and are structurally very similar. In contrast, the HCV helicase belongs to a different super-family of helicases, SF2, and shows little sequence homology with the PcrA/Rep helicases. Yet, the HCV helicase is structurally similar to Rep/PcrA, suggesting preservation of structural scaffolds and relationships between helicase motifs across these two super-families. The comparison study presented here also reveals the existence of a new helicase motif in the SF1 family of helicases.
摘要
最近已确定了三种解旋酶结构
DNA解旋酶PcrA的结构、丙型肝炎病毒RNA解旋酶的结构以及大肠杆菌DNA解旋酶Rep的结构。PcrA和Rep属于同一解旋酶超家族（SF1），在结构上非常相似。相比之下，HCV解旋酶属于不同的解旋酶超家族SF2，与PcrA/Rep解旋酶的序列同源性很低。然而，HCV解旋酶在结构上与Rep/PcrA相似，这表明在这两个超家族中解旋酶基序之间的结构支架和关系得以保留。此处呈现的比较研究还揭示了SF1解旋酶家族中存在一种新的解旋酶基序。
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