Majori M, Vachier I, Godard P, Farce M, Bousquet J, Chanez P
INSERM U454, Clinique des Maladies Respiratoires, Hôpital Arnaud de Villeneuve, Montpellier, France.
Eur Respir J. 1998 Jan;11(1):133-8. doi: 10.1183/09031936.98.11010133.
Reactive oxygen species play an important role in promoting inflammation. Blood monocytes have been described to release higher amounts of superoxide anion in uncontrolled and untreated asthmatics. Corticosteroids are widely used in asthma, but little is known about their molecular mechanism of action. The aim of our study was to analyse the ex vivo effect of corticosteroid treatments on superoxide anion release by blood monocytes. Superoxide anion release was measured by a spectrophotometric method based on the superoxide dismutase (SOD) -inhibitable reduction of ferricytochrome C by blood monocytes from untreated patients and asthmatics treated with i.v., inhaled and oral corticosteroids. Monocytes from uncontrolled and untreated asthmatics, released high amounts of superoxide anions. After short-term treatment with i.v., corticosteroids, this release was found to decrease significantly (1.410 versus 0.340 nM, p<0.05). Cells from asthmatics who had undergone long-term treatment with inhaled or oral steroids presented low amounts of superoxide anion production, with a significant difference as compared to untreated asthmatics (0.375 nM p<0.01 and 0.620 nM p<0.02 respectively). In general, patients with controlled asthma (treated with short-term oral steroids, or with long-term inhaled steroids) released lesser amounts of superoxide anion than uncontrolled and untreated asthmatics. In the case of steroid-dependent asthmatics there was no difference between patients with a controlled or uncontrolled disease.
活性氧在促进炎症方面发挥着重要作用。据描述，血液单核细胞在未得到控制和未经治疗的哮喘患者中会释放更多量的超氧阴离子。皮质类固醇广泛应用于哮喘治疗，但其作用的分子机制却鲜为人知。我们研究的目的是分析皮质类固醇治疗对血液单核细胞释放超氧阴离子的体外效应。通过分光光度法测量超氧阴离子的释放，该方法基于超氧化物歧化酶（SOD）抑制未治疗患者以及接受静脉注射、吸入和口服皮质类固醇治疗的哮喘患者的血液单核细胞对铁氰化细胞色素C的还原作用。未得到控制和未经治疗的哮喘患者的单核细胞释放大量超氧阴离子。静脉注射皮质类固醇进行短期治疗后，发现这种释放显著减少（1.410对0.340 nM，p<0.05）。接受吸入或口服类固醇长期治疗的哮喘患者的细胞产生的超氧阴离子量较低，与未治疗的哮喘患者相比有显著差异（分别为0.375 nM，p<0.01和0.620 nM，p<0.02）。一般来说，病情得到控制的哮喘患者（接受短期口服类固醇或长期吸入类固醇治疗）释放的超氧阴离子量比未得到控制和未经治疗的哮喘患者少。对于依赖类固醇的哮喘患者，病情得到控制或未得到控制的患者之间没有差异。
