Yoshida H, Tsujishita Y, Hullin F, Yoshida K, Nakamura S, Kikkawa U, Asaoka Y
Department of Biochemistry, Kobe University School of Medicine, Japan.
Ann Clin Biochem. 1998 Mar;35 ( Pt 2):295-301. doi: 10.1177/000456329803500216.
A Ca(2+)-independent phospholipase A that releases various fatty acids from sn-1 and sn-2 positions was partially purified from rat brain soluble fraction. The enzyme showed an approximate molecular mass of 300 kDa on gel filtration column chromatography. Its enzymatic properties are distinct from those of well characterized phospholipase A2 enzymes; by using a series of synthetic phosphatidylcholines, the enzyme cleaved oleic, linoleic, and arachidonic acids like phospholipase A2, and released palmitic and stearic acids like phospholipase A1. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidic acid were hydrolysed with almost equal efficiencies by this enzyme. These results indicate that the enzyme isolated is a novel Ca(2+)-independent intracellular phospholipase A that might be responsible for production of various fatty acids from membrane phospholipids.
从大鼠脑可溶性部分中部分纯化出一种不依赖钙离子的磷脂酶A，它能从sn-1和sn-2位释放各种脂肪酸。该酶在凝胶过滤柱色谱上显示出约300 kDa的分子量。其酶学性质与已充分表征的磷脂酶A2酶不同；通过使用一系列合成磷脂酰胆碱，该酶像磷脂酶A2一样裂解油酸、亚油酸和花生四烯酸，像磷脂酶A1一样释放棕榈酸和硬脂酸。该酶对磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酸的水解效率几乎相同。这些结果表明，分离出的该酶是一种新型的不依赖钙离子的细胞内磷脂酶A，可能负责从膜磷脂中产生各种脂肪酸。
