Lisowska-Myjak B, Pachecka J, Sokrates O, Brzozowska-Binda A, Torbicka E
Dept. of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Poland.
Scand J Gastroenterol. 1998 Mar;33(3):255-9. doi: 10.1080/00365529850170829.
The aim of this study was to compare the concentration and immunoelectrophoretic characterization of alpha-1-antitrypsin (AAT) excreted in random fecal samples in children with acute and chronic diarrhea and in control groups.
Thirty-two children with diarrhea and 23 healthy children were evaluated. The concentration and characterization of AAT were determined by radial immunodiffusion and crossed immunoelectrophoresis, respectively.
The increase in the concentration of fecal AAT was more than the upper limit for the control group (1.25 mg/g of dry stool mass) in the patients with chronic infectious diarrhea and in 52% of those with chronic non-infectious diarrhea but not in those with acute diarrhea, infectious or non-infectious. Immunoelectrophoretic analysis showed two forms of fecal AAT in both sick and healthy children. The alterations in the concentration of fecal AAT did not correlate with the immunoelectrophoretic pattern of AAT.
Our results suggest that the determination of fecal AAT could give clinically useful information about the difference between infectious and non-infectious diarrhea and the activity of characterizing disease with diarrhea.
本研究旨在比较急性和慢性腹泻患儿及对照组随机粪便样本中分泌的α-1抗胰蛋白酶（AAT）的浓度及免疫电泳特征。
对32例腹泻患儿和23例健康儿童进行评估。分别采用放射免疫扩散法和交叉免疫电泳法测定AAT的浓度和特征。
慢性感染性腹泻患儿及52%的慢性非感染性腹泻患儿粪便AAT浓度升高超过对照组上限（每克干粪便质量1.25毫克），但急性腹泻患儿（感染性或非感染性）粪便AAT浓度未升高。免疫电泳分析显示，患病儿童和健康儿童粪便中均有两种形式的AAT。粪便AAT浓度的变化与AAT的免疫电泳图谱无关。
我们的结果表明，测定粪便AAT可为区分感染性和非感染性腹泻以及腹泻疾病特征的活动提供临床有用信息。
