人胰岛素的一种新的半合成方法。

A new semisynthesis of human insulin.

作者信息

Obermeier R, Geiger R

出版信息

Hoppe Seylers Z Physiol Chem. 1976 Jun;357(6):759-67. doi: 10.1515/bchm2.1976.357.1.759.

DOI:10.1515/bchm2.1976.357.1.759

Abstract

A new semisynthesis of human insulin starting from porcine insulin is described. NalphaA1, NalphaB1-di-Boc-desoctapeptide-(B23-30)-insulin bearing 6 free carboxyl groups is coupled with the suitably protected human insulin-(B23-30)-octapeptide. The preferred reaction site is the carboxyl group of ArgB22. Side products are eliminated by extensive chromatographic purification.

摘要

描述了一种从猪胰岛素开始的人胰岛素新半合成方法。带有6个游离羧基的NαA1、NαB1 - 二 - Boc - 去八肽 - (B23 - 30) - 胰岛素与适当保护的人胰岛素 - (B23 - 30) - 八肽偶联。优选的反应位点是ArgB22的羧基。通过广泛的色谱纯化去除副产物。