CD4+、CD8+和CD4-CD8-T细胞亚群表达IL-18受体以及响应IL-18产生IFN-γ的差异能力。

Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18.

作者信息

Tomura M, Maruo S, Mu J, Zhou X Y, Ahn H J, Hamaoka T, Okamura H, Nakanishi K, Clark S, Kurimoto M, Fujiwara H

机构信息

Biomedical Research Center, Osaka University Medical School, Suita, Japan.

出版信息

J Immunol. 1998 Apr 15;160(8):3759-65.

DOI:

PMID:9558078

Abstract

IL-12 and IL-18 have the capacity to stimulate IFN-gamma production by T cells. Using a T cell clone, we reported that IL-18 responsiveness is generated only after exposure to IL-12. Here, we investigated the induction of IL-18 responsiveness in resting CD8+, CD4+, and CD4-CD8- T cells. Resting T cells respond to neither IL-12 nor IL-18. After stimulation with anti-CD3 plus anti-CD28 mAbs, CD8+, CD4+, and CD4-CD8- T cells expressed IL-12R, but not IL-18R, and produced IFN-gamma in response to IL-12. Cultures of T cells with anti-CD3/anti-CD28 in the presence of rIL-12 induced IL-18R expression and IL-18-stimulated IFN-gamma production, which reached higher levels than that induced by IL-12 stimulation. However, there was a substantial difference in the expression of IL-18R and IL-18-stimulated IFN-gamma production among T cell subsets. CD4+ cells expressed marginal levels of IL-18R and produced small amounts of IFN-gamma, whereas CD8+ cells expressed higher levels of IL-18R and produced more IFN-gamma than CD4+ cells. Moreover, CD4-CD8- cells expressed levels of IL-18R comparable to those for CD8+ cells but produced IFN-gamma one order higher than did CD8+ cells. These results indicate that the induction of IL-18R and IL-18 responsiveness by IL-12 represents a mechanism underlying enhanced IFN-gamma production by resting T cells, but the operation of this mechanism differs depending on the T cell subset stimulated.

摘要

白细胞介素-12（IL-12）和白细胞介素-18（IL-18）具有刺激T细胞产生γ干扰素（IFN-γ）的能力。我们利用一个T细胞克隆报道，只有在接触IL-12后，T细胞才会产生对IL-18的反应性。在此，我们研究了静息CD8⁺、CD4⁺和CD4⁻CD8⁻T细胞中IL-18反应性的诱导情况。静息T细胞对IL-12和IL-18均无反应。用抗CD3加抗CD28单克隆抗体刺激后，CD8⁺、CD4⁺和CD4⁻CD8⁻T细胞表达IL-12受体（IL-12R），但不表达IL-18受体（IL-18R），并对IL-12产生IFN-γ。在重组IL-12（rIL-12）存在的情况下，用抗CD3/抗CD28刺激T细胞培养物可诱导IL-18R表达和IL-18刺激的IFN-γ产生，其水平高于IL-12刺激所诱导的水平。然而，T细胞亚群之间在IL-18R表达和IL-18刺激的IFN-γ产生方面存在显著差异。CD4⁺细胞表达的IL-18R水平较低，产生的IFN-γ量较少，而CD8⁺细胞表达的IL-18R水平较高，产生的IFN-γ比CD4⁺细胞更多。此外，CD4⁻CD8⁻细胞表达的IL-18R水平与CD8⁺细胞相当，但产生的IFN-γ比CD错译：原文中“CD4-CD8- cells expressed levels of IL-18R comparable to those for CD8+ cells but produced IFN-gamma one order higher than did CD8+ cells.”的后半句翻译错误，“one order higher”应翻译为“高出一个数量级”，而不是“高出一级”。