单价琥珀酰伴刀豆球蛋白A的制备及其促有丝分裂活性

Preparation of monovalent succinyl-concanavalin A and its mitogenic activity.

作者信息

Beppu M, Terao T, Osawa T

出版信息

J Biochem. 1976 May;79(5):1113-7. doi: 10.1093/oxfordjournals.jbchem.a131152.

DOI:10.1093/oxfordjournals.jbchem.a131152

Abstract

Monovalent succinyl-concanavalin A was prepared by photoaffinity labeling of succinyl-concanavalin A with p-azidophenyl a-D-mannopyranoside. This concanavalin A derivate showed a weak but definite mitogenic acitivity against mouse splenic lymphocytes and human peripheral lymphocytes, suggesting that direct receptor cross-linkage on the cell surface is not an obligatory prerequisite for the activation of lymphocytes.

摘要

通过对琥珀酰伴刀豆球蛋白A与对叠氮苯基α-D-甘露吡喃糖苷进行光亲和标记制备了单价琥珀酰伴刀豆球蛋白A。这种伴刀豆球蛋白A衍生物对小鼠脾淋巴细胞和人外周淋巴细胞表现出微弱但明确的促有丝分裂活性，这表明细胞表面的直接受体交联并非淋巴细胞激活的必要前提条件。