金色链霉菌whiB下游的基因编码一种具有推测跨膜定位的大蛋白，且受葡萄糖诱导。

The gene downstream of Streptomyces aureofaciens whiB encodes a large protein with proposed transmembrane localization, and is induced by glucose.

作者信息

Homerová D, Sprusanský O, Potúcková L, Sevcíková B, Nováková R, Rezuchová B, Kormanec J

机构信息

Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 842 51 Bratislava, Slovak Republic.

出版信息

Biochim Biophys Acta. 1998 Apr 29;1397(2):151-5. doi: 10.1016/s0167-4781(98)00013-x.

DOI:10.1016/s0167-4781(98)00013-x

PMID:9565673

Abstract

The sequence analysis of the region downstream of the Streptomyces aureofaciens whiB sporulation gene revealed a long open reading frame (1219 amino acids; Mr 128 209) encoding protein with potential transmembrane structure. By integrative transformation, via double cross-over, a stable null mutant of the gene, orf1219, was prepared. This mutation appeared to have no obvious effect on vegetative growth and differentiation. In vitro and in vivo transcriptional analysis of the downstream gene revealed a single apparent promoter induced by glucose.

摘要

对金色链霉菌whiB孢子形成基因下游区域的序列分析揭示了一个长开放阅读框（1219个氨基酸；分子量128209），其编码的蛋白质具有潜在的跨膜结构。通过双交换的整合转化，制备了该基因orf1219的稳定缺失突变体。该突变似乎对营养生长和分化没有明显影响。对下游基因的体外和体内转录分析揭示了一个由葡萄糖诱导的单一明显启动子。