鉴定由冠状病毒传染性支气管炎病毒1a多聚蛋白经类3C蛋白酶加工而成的一种24 kDa多肽及其切割位点的确定。
Identification of a 24-kDa polypeptide processed from the coronavirus infectious bronchitis virus 1a polyprotein by the 3C-like proteinase and determination of its cleavage sites.
作者信息
Ng L F, Liu D X
机构信息
Institute of Molecular Agrobiology, Singapore.
出版信息
Virology. 1998 Apr 10;243(2):388-95. doi: 10.1006/viro.1998.9058.
Abstract
We report here the identification of a 24-kDa polypeptide in IBV-infected Vero cells by immunoprecipitation with a region-specific antiserum raised in rabbits against the IBV sequence encoded between nucleotides 10,928 and 11,493. Coexpression, deletion, and mutagenesis studies have demonstrated that this protein is encoded by ORF 1a from nucleotide 10,915 to 11,544 and is released from the 1a polyprotein by the 3C-like proteinase-mediated proteolysis. A previously predicted Q-S (Q3462S3463) dipeptide bond encoded by the IBV sequence from nucleotide 10,912 to 10,917 is identified as the N-terminal cleavage site, and a Q-N (Q3672N3673) dipeptide bond encoded by the IBV sequence between nucleotides 11,542 and 11,547 is identified as the C-terminal cleavage site of the 24-kDa polypeptide.
摘要
我们在此报告，通过用兔体内产生的针对传染性支气管炎病毒（IBV）核苷酸10928至11493之间编码序列的区域特异性抗血清进行免疫沉淀，在感染IBV的非洲绿猴肾细胞（Vero细胞）中鉴定出一种24 kDa的多肽。共表达、缺失和诱变研究表明，该蛋白由核苷酸10915至11544的开放阅读框1a（ORF 1a）编码，并通过3C样蛋白酶介导的蛋白水解作用从1a多蛋白中释放出来。由IBV核苷酸10912至10917编码的先前预测的Q-S（Q3462S3463）二肽键被确定为N端切割位点，而由IBV核苷酸11542至11547之间编码的Q-N（Q3672N3673）二肽键被确定为24 kDa多肽的C端切割位点。
相似文献
1
Identification of a 24-kDa polypeptide processed from the coronavirus infectious bronchitis virus 1a polyprotein by the 3C-like proteinase and determination of its cleavage sites.鉴定由冠状病毒传染性支气管炎病毒1a多聚蛋白经类3C蛋白酶加工而成的一种24 kDa多肽及其切割位点的确定。
Virology. 1998 Apr 10;243(2):388-95. doi: 10.1006/viro.1998.9058.
2
Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: identification of a 10-kilodalton polypeptide and determination of its cleavage sites.冠状病毒传染性支气管炎病毒1a多聚蛋白的蛋白水解加工：一种10千道尔顿多肽的鉴定及其切割位点的确定。
J Virol. 1997 Mar;71(3):1814-20. doi: 10.1128/JVI.71.3.1814-1820.1997.
3
Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products.冠状病毒传染性支气管炎病毒1b多聚蛋白的蛋白水解图谱分析：3C样蛋白酶存在四个切割位点的证据及两种新切割产物的鉴定
Virology. 1998 Jul 5;246(2):288-97. doi: 10.1006/viro.1998.9199.
4
Identification of a novel cleavage activity of the first papain-like proteinase domain encoded by open reading frame 1a of the coronavirus Avian infectious bronchitis virus and characterization of the cleavage products.鉴定冠状病毒禽传染性支气管炎病毒开放阅读框1a编码的首个木瓜蛋白酶样蛋白酶结构域的新型切割活性及切割产物的特性分析。
J Virol. 2000 Feb;74(4):1674-85. doi: 10.1128/jvi.74.4.1674-1685.2000.
5
Further identification and characterization of novel intermediate and mature cleavage products released from the ORF 1b region of the avian coronavirus infectious bronchitis virus 1a/1b polyprotein.对禽冠状病毒传染性支气管炎病毒1a/1b多聚蛋白ORF 1b区域释放的新型中间和成熟裂解产物的进一步鉴定与表征。
Virology. 2001 Sep 30;288(2):212-22. doi: 10.1006/viro.2001.1098.
6
Characterisation and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein.负责传染性支气管炎病毒1a/1b多聚蛋白蛋白水解加工的ORF 1a编码蛋白酶结构域的表征及突变分析。
Virology. 1995 Jun 1;209(2):420-7. doi: 10.1006/viro.1995.1274.
7
Further characterization of the coronavirus infectious bronchitis virus 3C-like proteinase and determination of a new cleavage site.冠状病毒传染性支气管炎病毒3C样蛋白酶的进一步特性分析及新切割位点的确定。
Virology. 2000 Jun 20;272(1):27-39. doi: 10.1006/viro.2000.0330.
8
A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products.由冠状病毒传染性支气管炎病毒开放阅读框（ORF）1b编码的一种100千道尔顿的多肽由ORF 1a产物加工而成。
J Virol. 1994 Sep;68(9):5772-80. doi: 10.1128/JVI.68.9.5772-5780.1994.
9
Proteolytic processing of the polyprotein encoded by ORF1b of the coronavirus infectious bronchitis virus (IBV).冠状病毒传染性支气管炎病毒（IBV）开放阅读框1b（ORF1b）编码的多聚蛋白的蛋白水解加工。
Adv Exp Med Biol. 1998;440:149-59. doi: 10.1007/978-1-4615-5331-1_20.
10
Further identification and characterization of products processed from the coronavirus avian infectious bronchitis virus (IBV) 1a polyprotein by the 3C-like proteinase.
Adv Exp Med Biol. 2001;494:291-8. doi: 10.1007/978-1-4615-1325-4_45.
引用本文的文献
1
Coronavirus RNA-dependent RNA polymerase interacts with the p50 regulatory subunit of host DNA polymerase delta and plays a synergistic role with RNA helicase in the induction of DNA damage response and cell cycle arrest in the S phase.冠状病毒 RNA 依赖性 RNA 聚合酶与宿主 DNA 聚合酶 δ 的 p50 调节亚基相互作用，并与 RNA 解旋酶协同作用，在诱导 S 期的 DNA 损伤反应和细胞周期停滞中发挥作用。
Emerg Microbes Infect. 2023 Dec;12(1):e2176008. doi: 10.1080/22221751.2023.2176008.
2
Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit.一种携带嵌合S1亚基的重组禽γ冠状病毒传染性支气管炎病毒的构建及其交叉保护效力
Front Microbiol. 2022 Jul 22;13:897560. doi: 10.3389/fmicb.2022.897560. eCollection 2022.
3
Interplay of the ubiquitin proteasome system and the innate immune response is essential for the replication of infectious bronchitis virus.泛素蛋白酶体系统与固有免疫反应的相互作用对于传染性支气管炎病毒的复制是必不可少的。
Arch Virol. 2021 Aug;166(8):2173-2185. doi: 10.1007/s00705-021-05073-3. Epub 2021 May 26.
4
Regulation of the ER Stress Response by the Ion Channel Activity of the Infectious Bronchitis Coronavirus Envelope Protein Modulates Virion Release, Apoptosis, Viral Fitness, and Pathogenesis.传染性支气管炎冠状病毒包膜蛋白的离子通道活性对内质网应激反应的调节作用，可调控病毒粒子释放、细胞凋亡、病毒适应性及发病机制。
Front Microbiol. 2020 Jan 24;10:3022. doi: 10.3389/fmicb.2019.03022. eCollection 2019.
5
The ER stress sensor IRE1 and MAP kinase ERK modulate autophagy induction in cells infected with coronavirus infectious bronchitis virus.内质网应激传感器 IRE1 和丝裂原活化蛋白激酶 ERK 调节感染冠状病毒传染性支气管炎病毒的细胞中自噬的诱导。
Virology. 2019 Jul;533:34-44. doi: 10.1016/j.virol.2019.05.002. Epub 2019 May 2.
6
Activation of the c-Jun NH-terminal kinase pathway by coronavirus infectious bronchitis virus promotes apoptosis independently of c-Jun.冠状病毒传染性支气管炎病毒通过激活 c-Jun NH-末端激酶通路促进细胞凋亡，而不依赖于 c-Jun。
Cell Death Dis. 2017 Dec 13;8(12):3215. doi: 10.1038/s41419-017-0053-0.
7
Coronavirus infectious bronchitis virus non-structural proteins 8 and 12 form stable complex independent of the non-translated regions of viral RNA and other viral proteins.冠状病毒感染性支气管炎病毒非结构蛋白8和12形成稳定复合物，不依赖于病毒RNA的非翻译区和其他病毒蛋白。
Virology. 2018 Jan 1;513:75-84. doi: 10.1016/j.virol.2017.10.004. Epub 2017 Oct 13.
8
Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture.冠状病毒传染性支气管炎病毒刺突蛋白中N-糖基化位点的鉴定及其对细胞培养中病毒复制和感染性的功能影响。
Virology. 2018 Jan 1;513:65-74. doi: 10.1016/j.virol.2017.10.003. Epub 2017 Oct 13.
9
Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus.传染性支气管炎病毒包膜蛋白中的通道失活突变及其回复突变体
J Virol. 2017 Feb 14;91(5). doi: 10.1128/JVI.02158-16. Print 2017 Mar 1.
10
Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus.中东呼吸综合征冠状病毒3C样蛋白酶假定切割位点的预测与生化分析
Virus Res. 2015 Oct 2;208:56-65. doi: 10.1016/j.virusres.2015.05.018. Epub 2015 May 31.
本文引用的文献
1
Identification, expression, and processing of an 87-kDa polypeptide encoded by ORF 1a of the coronavirus infectious bronchitis virus.冠状病毒传染性支气管炎病毒ORF 1a编码的87-kDa多肽的鉴定、表达及加工
Virology. 1995 Apr 1;208(1):48-57. doi: 10.1006/viro.1995.1128.
2
Identification and subcellular localization of a 41 kDa, polyprotein 1ab processing product in human coronavirus 229E-infected cells.人冠状病毒229E感染细胞中41 kDa多聚蛋白1ab加工产物的鉴定及其亚细胞定位
J Gen Virol. 1997 Nov;78 ( Pt 11):2789-94. doi: 10.1099/0022-1317-78-11-2789.
3
Formation of plant RNA virus replication complexes on membranes: role of an endoplasmic reticulum-targeted viral protein.植物RNA病毒复制复合体在膜上的形成：一种内质网靶向病毒蛋白的作用
EMBO J. 1997 Jul 1;16(13):4049-59. doi: 10.1093/emboj/16.13.4049.
4
Coxsackievirus protein 2B modifies endoplasmic reticulum membrane and plasma membrane permeability and facilitates virus release.柯萨奇病毒蛋白2B可改变内质网膜和质膜通透性，并促进病毒释放。
EMBO J. 1997 Jun 16;16(12):3519-32. doi: 10.1093/emboj/16.12.3519.
5
Identification of an ATPase activity associated with a 71-kilodalton polypeptide encoded in gene 1 of the human coronavirus 229E.鉴定与人冠状病毒229E基因1编码的71千道尔顿多肽相关的ATP酶活性。
J Virol. 1997 Jul;71(7):5631-4. doi: 10.1128/JVI.71.7.5631-5634.1997.
6
Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: identification of a 10-kilodalton polypeptide and determination of its cleavage sites.冠状病毒传染性支气管炎病毒1a多聚蛋白的蛋白水解加工：一种10千道尔顿多肽的鉴定及其切割位点的确定。
J Virol. 1997 Mar;71(3):1814-20. doi: 10.1128/JVI.71.3.1814-1820.1997.
7
Characterization of a 105-kDa polypeptide encoded in gene 1 of the human coronavirus HCV 229E.人冠状病毒HCV 229E基因1编码的105 kDa多肽的特性分析
Virology. 1996 Aug 1;222(1):227-35. doi: 10.1006/viro.1996.0413.
8
A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products.由冠状病毒传染性支气管炎病毒开放阅读框（ORF）1b编码的一种100千道尔顿的多肽由ORF 1a产物加工而成。
J Virol. 1994 Sep;68(9):5772-80. doi: 10.1128/JVI.68.9.5772-5780.1994.
9
Identification and characterization of a 65-kDa protein processed from the gene 1 polyprotein of the murine coronavirus MHV-A59.从小鼠冠状病毒MHV - A59基因1多聚蛋白加工而来的一种65 kDa蛋白的鉴定与特性分析
Virology. 1995 Feb 20;207(1):316-20. doi: 10.1006/viro.1995.1085.
10
Characterisation and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein.负责传染性支气管炎病毒1a/1b多聚蛋白蛋白水解加工的ORF 1a编码蛋白酶结构域的表征及突变分析。
Virology. 1995 Jun 1;209(2):420-7. doi: 10.1006/viro.1995.1274.
Zotero 插件