Okamoto Y, Kurane I, Leporati A M, Ennis F A
Center for Infectious Disease and Vaccine Research, Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
J Gen Virol. 1998 Apr;79 ( Pt 4):697-704. doi: 10.1099/0022-1317-79-4-697.
The epitopes recognized by six CD4+ CD8- cytotoxic T lymphocyte (CTL) clones established from a dengue-3 virus-immune donor were defined. (i) Three CTL clones, JK10, JK34 and JK39, were cross-reactive for dengue virus types 1-4. (ii) One clone, JK28, was cross-reactive for dengue virus types 1-4 and West Nile virus. (iii) Two clones, JK26 and JK49, were cross-reactive for dengue virus types 1-4, West Nile virus and yellow fever virus. The clones, except for JK49, recognized the same epitope on NS3 in an HLA-DPw2-restricted fashion. The smallest synthetic peptide recognized by the five CTL clones was a 10 aa peptide which comprises aa 255-264 on dengue virus NS3. JK49 recognized the overlapping epitope which comprises aa 257-266 in an HLA-DPw2-restricted fashion. Analysis of T cell receptor (TCR) usage by these T cell clones revealed that (i) JK10 and JK34 use V alpha11, and JK34 and JK28 use V beta23, and (ii) the amino acid sequences of the V(D)J junctional region of the TCR were different among these five CTL clones. There were, however, single amino acid conservations among TCRs of some of these T cell clones. These results indicate that the region on NS3 which comprises aa 255-266 contains multiple epitopes recognized by dengue serotype-cross-reactive and flavivirus-cross-reactive CD4+ CTL in an HLA-DPw2-restricted fashion and that a single epitope can be recognized by T cells which have heterogeneous virus specificities.
确定了从一名登革热3型病毒免疫供体中分离出的6个CD4+ CD8-细胞毒性T淋巴细胞（CTL）克隆所识别的表位。（i）3个CTL克隆JK10、JK34和JK39对1-4型登革热病毒具有交叉反应性。（ii）一个克隆JK28对1-4型登革热病毒和西尼罗河病毒具有交叉反应性。（iii）两个克隆JK26和JK49对1-4型登革热病毒、西尼罗河病毒和黄热病病毒具有交叉反应性。除JK49外，这些克隆以HLA-DPw2限制性方式识别NS3上的相同表位。5个CTL克隆识别的最小合成肽是一个10氨基酸肽，其包含登革热病毒NS3上的第255-264位氨基酸。JK49以HLA-DPw2限制性方式识别包含第257-266位氨基酸的重叠表位。对这些T细胞克隆的T细胞受体（TCR）使用情况分析表明：（i）JK10和JK34使用Vα11，JK34和JK28使用Vβ23；（ii）这5个CTL克隆的TCR的V（D）J连接区氨基酸序列不同。然而，这些T细胞克隆中的一些TCR之间存在单个氨基酸保守性。这些结果表明，NS3上包含第255-266位氨基酸的区域含有多个表位，这些表位以HLA-DPw2限制性方式被登革热血清型交叉反应性和黄病毒交叉反应性CD4+ CTL所识别，并且单个表位可被具有异源病毒特异性的T细胞所识别。
