外源Ca2+和Ca(2+)通道调节剂对红光诱导的菜豆原生质体肿胀的影响

The effect of external Ca2+ and Ca(2+)-channel modulators on red-light-induced swelling of protoplasts of Phaseolus radiatus L.

作者信息

Long C, Wang X J, Pan R C

机构信息

Department of Biology, South China Normal University, Guangzhou, China.

出版信息

Cell Res. 1998 Mar;8(1):41-50. doi: 10.1038/cr.1998.5.

DOI:10.1038/cr.1998.5

PMID:9570016

Abstract

Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean (Phaseolus radiatus L.) was observed only when Ca2+ ions were present in the medium. The optimal CaCl2 concentration was 250 microM. Swelling response declined when Ca2+ was supplied into the medium after red light irradiation. The Ca(2+)-chelator EGTA eliminated the red-light-induced swelling and 45Ca2+ accumulation in the protoplasts. In contrast, A23187, a Ca(2+)-ionophore, could mimic the effect of red light in darkness. These results indicate that Ca2+ may play a role in light signal transduction. In addition, swelling response was prevented by TFP and CPZ (both are CaM antagonists), implying the involvement of CaM in red-light-induced and Ca(2+)-dependent protoplast swelling.

摘要

只有当培养基中存在钙离子时，才会观察到红光诱导的从黄化绿豆（Phaseolus radiatus L.）下胚轴分离的原生质体肿胀。氯化钙的最佳浓度为250微摩尔。在红光照射后向培养基中供应钙离子时，肿胀反应下降。钙离子螯合剂乙二醇双四乙酸（EGTA）消除了红光诱导的原生质体肿胀和45钙离子积累。相反，钙离子载体A23187在黑暗中可模拟红光的作用。这些结果表明钙离子可能在光信号转导中起作用。此外，三氟拉嗪（TFP）和氯丙嗪（CPZ）（两者均为钙调蛋白拮抗剂）可阻止肿胀反应，这意味着钙调蛋白参与了红光诱导的和钙离子依赖性的原生质体肿胀。