Fujimura H A
Laboratory for Advanced Technology, Nippon Hoechst Marion Roussel, Kawagoe, Japan.
FEMS Microbiol Lett. 1998 Apr 15;161(2):359-64. doi: 10.1111/j.1574-6968.1998.tb12969.x.
Cells of the yeast Saccharomyces cerevisiae undergo morphogenesis in response to the mating pheromones a- and alpha-factors. The 'shmoo' morphology involves localized cell surface projection formation and cytoskeleton protein synthesis. This polarization is presumed to be a prelude to mating between mating partners with opposite mating types, a and alpha. To identify genes involved in pheromone-induced morphogenesis, a system in which a gpa1 fus3 double mutant was used as a parent strain was developed, and mutants which showed altered morphogenesis in response to mating pheromone were identified. The mutation was designated mgc1 for morphogenesis control by mating pheromones. The mgc1 mutant arrested cell division in response to alpha-factor and mated with cells of the opposite mating type, but did not form a typical projection in response to pheromones.
酿酒酵母细胞会响应交配信息素α-因子和a-因子而发生形态发生。“shmoo”形态涉及局部细胞表面突起的形成和细胞骨架蛋白的合成。这种极化被认为是具有相反交配类型a和α的交配伙伴之间交配的前奏。为了鉴定参与信息素诱导的形态发生的基因，开发了一种以gpa1 fus3双突变体作为亲本菌株的系统，并鉴定了对交配信息素表现出形态发生改变的突变体。该突变被命名为mgc1，代表由交配信息素控制的形态发生。mgc1突变体在响应α-因子时停止细胞分裂，并与相反交配类型的细胞交配，但对信息素不形成典型的突起。
