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弯曲杆菌属16S rRNA基因中的间隔序列:核苷酸序列的多样性和位置的一致性

Intervening sequences in 16S rRNA genes of Campylobacter sp.: diversity of nucleotide sequences and uniformity of location.

作者信息

Etoh Y, Yamamoto A, Goto N

机构信息

Department of Oral Microbiology, Showa University School of Dentistry, Tokyo, Japan.

出版信息

Microbiol Immunol. 1998;42(3):241-3. doi: 10.1111/j.1348-0421.1998.tb02278.x.

Abstract

We found and sequenced intervening sequences (IVSs) in the PCR-amplicons of 16S rRNA genes of 3 strains of Campylobacter rectus, 2 strains of C. curvus and 2 strains of C. sputorum. The lengths of the IVSs were 140 to 233 bp. The IVSs of C. rectus were identical and had a sequence homology of 55 to 79% against those of C. curvus and C. helveticus. The IVSs of C. sputorum were 97.9-100% homologous but poorly homologous to the other IVSs. In spite of the diversities of the lengths and the nucleotide sequences, all of the IVSs were located at the same position in the 16S rRNA genes.

摘要

我们在3株直肠弯曲杆菌、2株弯曲弯曲杆菌和2株唾沫弯曲杆菌的16S rRNA基因的PCR扩增子中发现并测序了间隔序列(IVS)。IVS的长度为140至233 bp。直肠弯曲杆菌的IVS是相同的,与弯曲弯曲杆菌和瑞士弯曲杆菌的IVS序列同源性为55%至79%。唾沫弯曲杆菌的IVS同源性为97.9 - 100%,但与其他IVS的同源性较差。尽管长度和核苷酸序列存在差异,但所有IVS都位于16S rRNA基因的同一位置。

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