Li X M, Schofield B H, Wang Q F, Kim K H, Huang S K
Johns Hopkins Asthma and Allergy Center, School of Medicine, The Johns Hopkins University, Baltimore, MD 21224, USA.
J Immunol. 1998 Feb 1;160(3):1378-84.
The development of pulmonary allergic responses was examined in mice following pulmonary transfer of Ag (conalbumin)-specific Th2 cells. The levels of serum-specific IgE, cellular infiltrates, airway mucus goblet cells, and airway responsiveness were analyzed and compared with those in Ag-sensitized and -challenged mice. Pulmonary transfer of the conalbumin-specific Th2 clone (D10) induced, in an Ag-specific manner, high levels of the Th2 cytokines IL-4 and IL-5 in the bronchoalveolar lavage fluids and mucosal eosinophils, concomitant with an increase in airway responsiveness. The D10 cell-induced responses were seen in the absence of serum specific IgE. In the presence of Ag, the transferred D10 cells not only remained in the lungs, but also increased in number 72 h post-cell transfer. Although significantly higher levels of IL-4 and IL-5 in the bronchoalveolar lavage fluids were found in D10-transferred mice, the levels of pulmonary eosinophilia, mucus goblet cells, and airway responsiveness were significantly lower than those in Ag-sensitized and -challenged mice. These results demonstrate that although Ag-specific activation of Th2 cells at mucosal sites is able to mediate the recruitment of eosinophils and the subsequent induction of airway hyper-responsiveness, the more severe pulmonary allergic responses were observed only in mice sensitized and challenged with Ag.
在将抗原（伴清蛋白）特异性Th2细胞经肺转移至小鼠后，检测了肺部过敏反应的发展情况。分析了血清特异性IgE水平、细胞浸润、气道黏液杯状细胞和气道反应性，并与抗原致敏和激发的小鼠进行了比较。伴清蛋白特异性Th2克隆（D10）经肺转移以抗原特异性方式在支气管肺泡灌洗液和黏膜嗜酸性粒细胞中诱导产生高水平的Th2细胞因子IL-4和IL-5，同时气道反应性增加。在没有血清特异性IgE的情况下也能观察到D10细胞诱导的反应。在有抗原存在的情况下，转移的D10细胞不仅留在肺中，而且在细胞转移后72小时数量增加。虽然在D10细胞转移的小鼠支气管肺泡灌洗液中发现IL-4和IL-5水平显著更高，但肺部嗜酸性粒细胞增多、黏液杯状细胞和气道反应性水平显著低于抗原致敏和激发的小鼠。这些结果表明，虽然黏膜部位Th2细胞的抗原特异性激活能够介导嗜酸性粒细胞的募集和随后气道高反应性的诱导，但只有在经抗原致敏和激发的小鼠中才观察到更严重的肺部过敏反应。
