两种韩国甘薯羽状斑驳马铃薯Y病毒分离株外壳蛋白基因的核苷酸序列分析

Nucleotide sequence analysis of the coat protein genes of two Korean isolates of sweet potato feathery mottle potyvirus.

作者信息

Ryu K H, Kim S J, Park W M

机构信息

Department of Plant Pathology, Cornell University, Ithaca, New York, USA.

出版信息

Arch Virol. 1998;143(3):557-62. doi: 10.1007/s007050050311.

DOI:10.1007/s007050050311

PMID:9572555

Abstract

The coat protein (CP) genes of the genomic RNA of two Korean isolates of sweet potato feathery mottle potyvirus (SPFMV), SPFMV-K1 and SPFMV-K2, were cloned and their complete nucleotide sequences were determined. Sequence comparisons of the two Korean isolates showed 97.8% amino acid identity in the CP cistron, and 79.9% to 99.0% identity with those of 6 other known SPFMV strains. Of 74 amino acid changes totally among the SPFMV strains, 39 changes were located at the N-terminal region. Pairwise amino acid sequence comparison revealed sequence similarities of 48.6 to 70.2% between SPFMV and 20 other potyviruses, indicating SPFMV to be a quite distinct species. Multiple alignment of the CP cistrons from other potyviruses showed that most of the conserved amino acid residues of the genus Potyvirus are well preserved in the corresponding locations.

摘要

克隆了韩国甘薯羽状斑驳马铃薯Y病毒（SPFMV）两个分离株SPFMV-K1和SPFMV-K2基因组RNA的外壳蛋白（CP）基因，并测定了它们的完整核苷酸序列。对这两个韩国分离株的序列比较显示，CP顺反子中的氨基酸同一性为97.8%，与其他6个已知SPFMV株系的同一性为79.9%至99.0%。在SPFMV株系中总共74个氨基酸变化中，39个变化位于N端区域。成对氨基酸序列比较显示，SPFMV与其他20种马铃薯Y病毒之间的序列相似性为48.6%至70.2%，表明SPFMV是一个相当独特的种。来自其他马铃薯Y病毒的CP顺反子的多重比对表明，马铃薯Y病毒属的大多数保守氨基酸残基在相应位置保存良好。