雌激素可下调大鼠垂体中编码胞吐蛋白SNAP-25的信使核糖核酸。

Estrogen down-regulates mRNA encoding the exocytotic protein SNAP-25 in the rat pituitary gland.

作者信息

Jacobsson G, Razani H, Ogren S O, Meister B

机构信息

Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.

出版信息

J Neuroendocrinol. 1998 Mar;10(3):157-63. doi: 10.1046/j.1365-2826.1998.00178.x.

DOI:10.1046/j.1365-2826.1998.00178.x

PMID:9576603

Abstract

Exocytosis is dependent on specific proteins that are located at the secretory granule membrane, in the cytoplasm or at the plasma membrane. The mRNA expression of synaptosomal-associated protein of 25 kDa (SNAP-25) isoforms SNAP-25a and SNAP-25b, vesicle associated membrane protein (VAMP) 2, mammalian homologue of unc-18 (munc-18) and Hrs-2 was studied in the pituitary of ovariectomized rats after subcutaneous insertion of capsules containing estrogen or placebo using in situ hybridization. Estrogen treatment (0.25 mg estradiol) significantly decreased SNAP-25a (32%; 10%) and SNAP-25b (25%; 22%) mRNA levels in the anterior and intermediate lobes, respectively, whereas VAMP-2, munc-18 and Hrs-2 mRNA levels remained unchanged. The results suggest that estrogen selectively regulates SNAP-25 transcription in the pituitary gland, but leaves VAMP-2, munc-18 and Hrs-2 mRNA levels unaffected.

摘要

胞吐作用依赖于位于分泌颗粒膜、细胞质或质膜上的特定蛋白质。使用原位杂交技术，研究了皮下植入含雌激素或安慰剂胶囊的去卵巢大鼠垂体中25 kDa突触体相关蛋白（SNAP-25）亚型SNAP-25a和SNAP-25b、囊泡相关膜蛋白（VAMP）2、unc-18哺乳动物同源物（munc-18）和Hrs-2的mRNA表达。雌激素处理（0.25 mg雌二醇）分别使前叶和中叶的SNAP-25a（32%；10%）和SNAP-25b（25%；22%）mRNA水平显著降低，而VAMP-2、munc-18和Hrs-2的mRNA水平保持不变。结果表明，雌激素选择性调节垂体中SNAP-25的转录，但不影响VAMP-2、munc-18和Hrs-2的mRNA水平。