PP7是一种植物磷酸酶，代表真核蛋白丝氨酸/苏氨酸磷酸酶的一个新的进化分支。

PP7, a plant phosphatase representing a novel evolutionary branch of eukaryotic protein Ser/Thr phosphatases.

作者信息

Andreeva A V, Evans D E, Hawes C R, Bennett N, Kutuzov M A

机构信息

Research School of Biological and Molecular Sciences, Oxford Brookes University, UK.

出版信息

Biochem Mol Biol Int. 1998 Apr;44(4):703-15. doi: 10.1080/15216549800201752.

DOI:10.1080/15216549800201752

PMID:9584984

Abstract

We describe a novel protein Ser/Thr phosphatase from Arabidopsis thaliana, PP7, which is only 27-32% identical in amino acid sequence to the known phosphatases and is the most divergent member of the PPP (PP1/2A/2B) family for today. Some structural features suggest more close relationship of PP7 to the PP5/rdgC subfamily. PP7 contains all of the residues essential for the phosphatase activity and possesses three major insertions in its presumable C-terminal subdomain, which suggest its unique regulation and/or optimisation of its structure for interaction with specific substrates or regulators. A phosphatase structurally related to PP7 is expressed in rice. PP7 conservation between mono- and dicotyledonous plants may point to its essential role in the plant cell.

摘要

我们描述了一种来自拟南芥的新型蛋白质丝氨酸/苏氨酸磷酸酶PP7，其氨基酸序列与已知磷酸酶的同源性仅为27%-32%，是目前PPP（PP1/2A/2B）家族中差异最大的成员。一些结构特征表明PP7与PP5/rdgC亚家族的关系更为密切。PP7包含磷酸酶活性所必需的所有残基，并且在其可能的C末端亚结构域中有三个主要插入片段，这表明其具有独特的调节方式和/或为与特定底物或调节因子相互作用而优化的结构。一种与PP7结构相关的磷酸酶在水稻中表达。单子叶植物和双子叶植物之间PP7的保守性可能表明其在植物细胞中起着重要作用。