Chiang J M
Division of Colon and Rectal Surgery, Chang Gung Memorial Hospital, Taipei, Taiwan.
Cancer Lett. 1998 Apr 24;126(2):179-85. doi: 10.1016/s0304-3835(98)00008-1.
In order to investigate whether two common types of colorectal cancer with different gross appearances share the same incidence of K-ras mutation, the occurrence of a point mutation in codon 12 or codon 13 of the K-ras oncogene was determined in 50 cases of colorectal carcinoma. The PCR technique was employed, followed by RFLP analysis and sequencing to identify specific point mutations. No correlation was found between the presence of a K-ras gene mutation and histological parameters. A strong association was found between K-ras mutations and the gross polypoid appearance of colorectal carcinoma (polypoid 73% versus ulcerative 8%). The results indicate a preferential association with the K-ras oncogene involved in polypoid type colorectal carcinoma.
为了研究两种具有不同大体外观的常见结直肠癌是否具有相同的K-ras突变发生率，对50例结直肠癌患者的K-ras癌基因第12或13密码子的点突变情况进行了测定。采用聚合酶链反应（PCR）技术，随后进行限制性片段长度多态性（RFLP）分析和测序以鉴定特定的点突变。未发现K-ras基因突变与组织学参数之间存在相关性。发现K-ras突变与结直肠癌的大体息肉样外观之间存在密切关联（息肉样73%，溃疡性8%）。结果表明，K-ras癌基因与息肉样类型的结直肠癌存在优先关联。
