Nakashima S, Matsuura N, Nagatsugi F, Maeda M, Sasaki S
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Nucleic Acids Symp Ser. 1997(37):33-4.
Regulation of specific gene expression by the sequence specific triplex formation has been expected as a new biological tool or a new therapeutic method in the so-called antigene strategy. As native nucleobases form triplexes only within homopurine-homopyrimidine duplexes, expansion of the target sequences to general ones has been desired. We have previously reported the selective formation of base triplets by the non-native nucleobases (1, 2) with a CG or a TA base pair, respectively, in the organic solvent. In this paper, we wish to report the synthesis of the new non-native bases (3, 4, 9-11) and their incorporation into the triplex-forming oligonucleotides (TFOs), as well as their ability of triplex formation.
通过序列特异性三链体形成来调控特定基因表达，在所谓的反基因策略中有望成为一种新的生物学工具或新的治疗方法。由于天然核碱基仅在同型嘌呤-同型嘧啶双链体中形成三链体，因此人们一直期望将靶序列扩展到一般序列。我们之前曾报道过，在有机溶剂中，非天然核碱基（1, 2）分别与CG或TA碱基对选择性形成碱基三联体。在本文中，我们希望报告新的非天然碱基（3, 4, 9 - 11）的合成及其掺入三链体形成寡核苷酸（TFO）的情况，以及它们形成三链体的能力。
