Solid phase synthesis of U1 RNA oligomers containing a TMG cap structure.
作者信息
Kadokura M, Ushioda M, Wada T, Sekine M
机构信息
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):77-8.
PMID:9586007
Abstract
We report the solid-phase synthesis of a 5'-terminal part of U1 RNA. In this approach, a new method for pyro- and tri-phosphate bond formation on solid supports was studied. Since 2,2,7-trimethyl guanosine (TMG) in the cap structure was unstable under basic conditions, a RNA oligomer was synthesized by using a linker having the P-N bond, which can be cleaved from the solid support by treatment with 80% acetic acid.
摘要
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