dCTP反馈抑制对小鼠脱氧胞苷激酶的手性影响。

Chiral influences of feedback inhibition with dCTP on murine deoxycytidine kinase.

作者信息

Tomikawa A, Yamaguchi T, Kawaguchi T, Shudo K, Saneyoshi M

机构信息

Department of Biological Sciences, Teikyo University of Science and Technology, Yamanashi, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):181-2.

PMID:9586059

Abstract

The inhibitory effects of 4 kinds of 2'-deoxy-L-nucleoside 5'-triphosphates, which are enantiomers of natural dNTPs, on murine deoxycytidine kinase (dCK) were investigated. When ATP was used as the phosphate donor, L-dCTP showed significant inhibitory action noncompetitively and competitively with 2'-deoxycytidine (dCyd) and ATP, respectively. Thus L-dCTP, like dCTP, could serve as a feedback inhibitor for dCK. Recently, it has been demonstrated that human dCK can utilize L-dCyd as a substrate (Verri, A. et al. (1997) Mol. Pharmacol., 51, 132). The present results suggest that dCK is also unable to discriminate the chirality of nucleotides at the phosphate donor binding site of the enzyme.

摘要

研究了4种作为天然脱氧核苷三磷酸（dNTP）对映体的2'-脱氧-L-核苷5'-三磷酸对小鼠脱氧胞苷激酶（dCK）的抑制作用。当以ATP作为磷酸供体时，L-dCTP分别与2'-脱氧胞苷（dCyd）和ATP非竞争性和竞争性地表现出显著的抑制作用。因此，L-dCTP与dCTP一样，可作为dCK的反馈抑制剂。最近，已证明人dCK能够利用L-dCyd作为底物（Verri，A.等人（1997年），《分子药理学》，51，132）。目前的结果表明，dCK在该酶的磷酸供体结合位点也无法区分核苷酸的手性。