Iwase R, Tsuchida H, Yamaoka T, Yamana K, Murakami A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Nucleic Acids Symp Ser. 1997(37):205-6.
Fluorescent-labeled oligonucleotides containing 2'-(1-pyrenylmethyl) uridine (Upy) that have G or C adjacent to 3'-site of Upy, I and II, respectively, showed a remarkable increase in the emission intensity when they hybridized with the complementary oligoribonucleotides (ORN). However such change was not observed when they hybridized with the complementary oligodeoxyribonucleotides(ODN). Tm values derived from the UV melting curves for I-ORN and II-ORN duplexes were 5 degrees C and 10 degrees C lower than the normal duplexes without Upy, respectively. The intensity of induced CD based on the pyrene groups for the I-ORN and II-ORN duplexes were a half of that for the corresponding I-ODN and II-ODN duplexes. These results suggest that pyrene groups were released from the stacking interaction upon duplex formation and that the remarkable change of emission intensity was then occurred. Based on these properties, it is suggested that Upy-probes that have G or C adjacent to 3'-site of Upy can be useful to search the single strand region of RNA.
含有2'-(1-芘甲基)尿苷(Upy)的荧光标记寡核苷酸，在Upy的3'位点分别与G或C相邻，即I和II，当它们与互补的寡核糖核苷酸(ORN)杂交时，发射强度显著增加。然而，当它们与互补的寡脱氧核糖核苷酸(ODN)杂交时，未观察到这种变化。I-ORN和II-ORN双链体的紫外熔解曲线得出的Tm值分别比不含Upy的正常双链体低5摄氏度和10摄氏度。基于芘基团的I-ORN和II-ORN双链体的诱导圆二色性强度是相应的I-ODN和II-ODN双链体的一半。这些结果表明，芘基团在双链体形成时从堆积相互作用中释放出来，随后发生发射强度的显著变化。基于这些特性，有人提出，在Upy的3'位点与G或C相邻的Upy探针可用于搜索RNA的单链区域。
