毛细管区带电泳法测定某些抗阿尔茨海默病药物

Capillary zone electrophoretic determination of some drugs against Alzheimer's disease.

作者信息

Vargas M G, Havel J, Patocka J

机构信息

Department of Analytical Chemistry, Faculty of Sciences, Masaryk University, Brno, Czech Republic.

出版信息

J Chromatogr A. 1998 Apr 3;802(1):121-8. doi: 10.1016/s0021-9673(97)01117-5.

DOI:10.1016/s0021-9673(97)01117-5

PMID:9588015

Abstract

A new capillary zone electrophoresis (CZE) method for the determination of tacrine (THA), 7-methoxytacrine (7-MTHA) and their basic metabolites (THAm, 7-MTHAm) in pharmaceutical and biological samples (urine and serum) was developed. Separation of all compounds by CZE was carried out using a 46.6 cm untreated fused-silica capillary applying 20 kV separation voltage using 50 mM phosphate buffer of pH 2.8 for THA and THAm and of pH 7.8 for 7-MTHA and 7-MTHAm as background electrolyte (BGE). Detection was carried out at 240 nm (THA and THAm) and 248 nm (7-MTHA and 7-MTHAm). THA and THAm were separated in less than 4 min while 7-MTHA and 7-MTHAm were separated in less than 7 min. The detection limits (SIN = 3) obtained were 3 ppb for THA and 4 ppb for 7-MTHA in aqueous solutions; 50 ppb for THA and 47 ppb for 7-MTHA for the determination in urine (diluted 1:10); 52 ppb for THA and 56 ppb for 7-MTHA, in deproteinized serum samples. The methods are suitable for therapeutic drug monitoring of the drugs.

摘要

建立了一种新的毛细管区带电泳（CZE）方法，用于测定药物和生物样品（尿液和血清）中的他克林（THA）、7-甲氧基他克林（7-MTHA）及其碱性代谢产物（THAm、7-MTHAm）。采用46.6 cm未处理的熔融石英毛细管进行CZE分离，以50 mM pH 2.8的磷酸盐缓冲液用于THA和THAm、pH 7.8的磷酸盐缓冲液用于7-MTHA和7-MTHAm作为背景电解质（BGE），施加20 kV分离电压。检测在240 nm（THA和THAm）和248 nm（7-MTHA和7-MTHAm）处进行。THA和THAm在不到4分钟内分离，而7-MTHA和7-MTHAm在不到7分钟内分离。在水溶液中获得的检测限（S/N = 3），THA为3 ppb，7-MTHA为4 ppb；在尿液（稀释1:10）中测定时，THA为50 ppb，7-MTHA为47 ppb；在脱蛋白血清样品中，THA为52 ppb，7-MTHA为56 ppb。这些方法适用于这些药物的治疗药物监测。