Pillay U, Kramer B
Department of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
J Dent Assoc S Afr. 1997 Nov;52(11):673-7.
The polymerase chain reaction (PCR) has been used to amplify a region of the ZFX and ZFY genes from DNA in human blood and other tissues, for determination of the sex of an individual. In the present study DNA was extracted from the pulp of 21 male and 24 female fresh human third molar teeth. A region of the ZFX and ZFY genes was amplified by PCR and analysed by digestion of the amplified DNA with HaeIII restriction endonuclease. The digested PCR products were run on a 2 per cent agarose gel. Males were distinguished from females by having a fragment of 317 base pairs which was absent in females. Identification of the sex of the individual was 100 per cent accurate in each case. A blind study of random samples of the same teeth, used to assess the reproducibility of the technique evoked an identical result. This method provides an accurate alternative to skeletal measurements and histological staining techniques for the sexing of individuals from small amounts of DNA.
聚合酶链反应（PCR）已被用于从人类血液和其他组织的DNA中扩增ZFX和ZFY基因的一个区域，以确定个体的性别。在本研究中，从21颗男性和24颗女性新鲜人类第三磨牙的牙髓中提取DNA。通过PCR扩增ZFX和ZFY基因的一个区域，并用HaeIII限制性内切酶消化扩增的DNA进行分析。消化后的PCR产物在2%的琼脂糖凝胶上进行电泳。男性与女性的区别在于男性有一个317个碱基对的片段，而女性没有。在每种情况下，个体性别的鉴定准确率均为100%。对相同牙齿的随机样本进行的盲法研究，用于评估该技术的可重复性，得到了相同的结果。这种方法为从小量DNA中确定个体性别提供了一种准确的替代骨骼测量和组织学染色技术的方法。
Zotero 插件