Kotani T
Department of Laboratory Medicine, Miyazaki Medical College.
Rinsho Byori. 1998 Apr;46(4):324-30.
Thyroid peroxidase (TPO) is a member of the thyroid autoantigens. Anti-thyroid autoantibodies, anti-TPO,-thyroglobulin and -TSH receptor autoantibodies, are important in diagnosing autoimmune thyroid diseases and for judging treatment efficacy. To diagnose chronic thyroiditis, anti-TPO autoantibody detection is the most sensitive among the three anti-thyroid autoantibodies. Eighty-five to ninety percent of patients with chronic thyroiditis have anti-TPO autoantibodies. Using recombinant human TPO protein, a kit measuring anti-TPO antibodies specifically have been developed. The developmental process and specificity of the kit are discussed. Compared with the microsome test, the most greatest advantage of the anti-TPO EIA kit can specifically measure anti-TPO antibodies. Structure of anti-TPO antibody immunoglobulin, which has been demonstrated over the last several years using the phage display method, is also discussed. The most prominent feature is that VL is believed to be important in determining anti-TPO antibody specificity in contrast to that of other autoantibodies, although VL itself does not have TPO-binding activity.
甲状腺过氧化物酶（TPO）是甲状腺自身抗原的一种。抗甲状腺自身抗体，如抗TPO、抗甲状腺球蛋白和抗促甲状腺激素受体自身抗体，在自身免疫性甲状腺疾病的诊断及判断治疗效果方面具有重要意义。在诊断慢性甲状腺炎时，抗TPO自身抗体检测是三种抗甲状腺自身抗体中最敏感的。85%至90%的慢性甲状腺炎患者存在抗TPO自身抗体。利用重组人TPO蛋白，已开发出一种专门检测抗TPO抗体的试剂盒。本文讨论了该试剂盒的研发过程及特异性。与微粒体检测相比，抗TPO酶免疫分析试剂盒最大的优势在于能够特异性地检测抗TPO抗体。本文还讨论了过去几年利用噬菌体展示法所证实的抗TPO抗体免疫球蛋白的结构。最显著的特点是，与其他自身抗体不同，尽管VL本身不具有TPO结合活性，但据信VL在决定抗TPO抗体特异性方面起着重要作用。
