蛋白质合成在促黄体生成素（LH）刺激大鼠体外排卵前卵泡中前列腺素积累过程中的作用。

The role of protein synthesis in the stimulation by LH of prostaglandin accumulation in rat preovulatory follicles in vitro.

作者信息

Clark M R, Marsh J M, LeMaire W J

出版信息

Prostaglandins. 1976 Aug;12(2):209-16. doi: 10.1016/0090-6980(76)90116-7.

Abstract

Preovulatory follicles isolated from immature rats, treated in vivo with pregnant mare's serum gonadotropin, were incubated in vitro and the accumulation of prostaglandin E measured. The addition of luteinizing hormone (5 mug/ml) increased this accumulation, after a lag period of 3 hours. This delay suggested the involvement of macromolecular synthesis in the mechanism of prostaglandin stimulation by luteinizing hormone. When the synthesis of protein was inhibited by the addition of puromycin (100 muM), the luteinizing hormone stimulation of prostaglandin E in these follicles was completely abolished. This inhibition was not seen with an analogue of puromycin, which does not inhibit protein synthesis, puromycin amino-nucleoside. These data suggest that concomitant protein synthesis is required for the luteinzing hormone stimulation of prostaglandin accumulation in rat follicles.

摘要

从用孕马血清促性腺激素进行体内处理的未成熟大鼠中分离出排卵前卵泡，进行体外培养并测定前列腺素E的积累量。添加促黄体生成素（5微克/毫升）后，经过3小时的延迟期，前列腺素E的积累量增加。这种延迟表明大分子合成参与了促黄体生成素刺激前列腺素生成的机制。当添加嘌呤霉素（100微摩尔）抑制蛋白质合成时，促黄体生成素对这些卵泡中前列腺素E的刺激作用完全被消除。而不抑制蛋白质合成的嘌呤霉素类似物嘌呤霉素氨基核苷则未观察到这种抑制作用。这些数据表明，促黄体生成素刺激大鼠卵泡中前列腺素积累需要伴随蛋白质合成。