[结核分枝杆菌链霉素耐药基因的检测]

[Detection of M. tuberculosis streptomycin-resistant gene].

作者信息

Wu X, Zhuang Y, He X

机构信息

Tuberculosis Research Laboratory, 309th Hospital, PLA, Beijing.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 1996 Dec;19(6):342-5.

PMID:9596815

Abstract

OBJECTIVE

To observe the mutations of rpsL gene in M. tuberculosis streptomycin-resistant isolates, and to develop a new method for detecting drug resistance.

METHOD

Detecting the rpsL genes with PCR-SSCP and analyzing their codons 43 by PCR-RFLP.

RESULTS

Strain H37Rv was used as a control. In 22 M. tuberculosis clinical isolates, the rpsL PCR fragments from 5 drug-susceptible isolates had no differences in the SSCP profiles with strain H37Rv, and were restricted by Mbo II. 11 of the 13 streptomycin-resistant isolates showed apparent differences in the SSCP profiles and were not digested with Mbo II. 1 of the 4 other drug-resistant isolates also had apparent SSCP differences and was not digested by Mbo II.

CONCLUSIONS

The results suggested that the rpsL gene mutation was frequently observed in M. tuberculosis streptomycin-resistant isolates, and usually situated at codon 43, PCR-SSCP and PCR-RFLP might become a simple, rapid and reliable diagnostic test for drug resistance.

摘要

目的

观察结核分枝杆菌耐链霉素分离株中rpsL基因的突变情况，建立一种新的耐药检测方法。

方法

采用PCR-SSCP检测rpsL基因，并通过PCR-RFLP分析其第43位密码子。

结果

以H37Rv菌株作为对照。在22株结核分枝杆菌临床分离株中，5株药敏菌株的rpsL基因PCR片段在SSCP图谱上与H37Rv菌株无差异，且能被Mbo II酶切。13株耐链霉素分离株中有11株在SSCP图谱上表现出明显差异，且不能被Mbo II酶切。另外4株耐药分离株中有1株在SSCP图谱上也有明显差异，且不能被Mbo II酶切。