[结核分枝杆菌中异烟肼耐药性与katG突变的关系]
[Relationship between INH-resistance and katG mutation in M. tuberculosis].
作者信息
Zhang Z, Ma Y, Cheng S
机构信息
Beijing Tuberculosis and Thoracic Tumor Institute..
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 1996 Dec;19(6):346-9.
OBJECTIVE
To investigate the molecular mechanism of INH-resistance and the relationship between INH-resistance and KatG mutations.
METHODS
Use the primers designed from the 5' end of the KatG gene to amplify 282bp fragments from INH-susceptible and -resistant M. tuberculosis isolates. Single strand conformation polymorphism (SSCP) for their PCR products were analysed.
RESULTS
No katG gene sequence deletion was observed, mutations were detected in 17 of 30 INH-resistant strains and no gene perturbations were shown in 16 INH-susceptible isolates.
CONCLUSION
The results suggested that mutation in katG gene was one of the most important INH-resistant mechanisms in M. tuberculosis.
目的
探讨异烟肼耐药的分子机制以及异烟肼耐药与KatG基因突变之间的关系。
方法
使用从KatG基因5'端设计的引物，从异烟肼敏感和耐药的结核分枝杆菌分离株中扩增282bp片段。对其PCR产物进行单链构象多态性（SSCP）分析。
结果
未观察到katG基因序列缺失，在30株异烟肼耐药菌株中有17株检测到突变，16株异烟肼敏感分离株未显示基因扰动。
结论
结果表明，katG基因突变是结核分枝杆菌最重要的异烟肼耐药机制之一。
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