Zhao X, Zhang Y, Su Y
Shandong Provincial Hospital, Jinan.
Zhonghua Fu Chan Ke Za Zhi. 1997 Apr;32(4):217-21.
To establish a xenografted tumor strain of human cervical carcinoma in nude mice and homologous cell line in vitro.
A human well differentiated cervical squamous cell carcinoma derived from a surgical specimen was transplanted in the subcutis of nude mice. The xenograft was cultured to establish a cell line in vitro. A series of characteristics of the grafts and the cells were examined. The established cell line was cloned by single cell detaching flask. Non-serum culture of the cells was tried.
The xenografts were successively passaged for 26 generations during a period of 17 months. A tumor strain HCC-94V was established, having a high rate of successful inoculation rate (92.9%) and was growing steadily. The morphological and biological characteristics of its original tumor were preserved. The chromosomal analysis revealed a human aneuploid pattern of 9-120 in number. The tumor markers detected by immunocytochemical techniques showed high expression, the oncogenic products, low expression. Polymerase chain reaction (PCR) showed the same results as those of the patients with original tumor tissue, human papilloma virus (HPV) 16 (+), 18 (-). A new human cell line HCC-94 derived from the xenograft, was established and maintained for 16 months through 131 passages. Transmission electromicroscopy demonstrated the presence of typical desmosomes between the cells and many tonofilaments in the cells. Also, the morphology of chromosomes showed the characteristics of chromosomes as well as of mutants in the human tumor cells. The tumor markers and the oncogenic products of the cells exhibited higher expression than that of the original tumor. The HPV 16 and 18 of the cells, using PCR, were negative. The cells were highly tumorigenic in nude mice. The transplanted tumors resembled the original tumor tissues in morphology and were HPV 16 positive. Both cell cloning culture and non-serum culture of HCC-94 were successful.
HCC-94V and HCC-94 could be considered as a new xenografted tumor strain may provide material and model ideal for further investigation of human cervical carcinoma.
在裸鼠中建立人宫颈癌异种移植瘤株并在体外建立同源细胞系。
将来源于手术标本的人高分化宫颈鳞状细胞癌移植到裸鼠皮下。对异种移植瘤进行培养以在体外建立细胞系。检测移植瘤和细胞的一系列特性。通过单细胞分离瓶对建立的细胞系进行克隆。尝试对细胞进行无血清培养。
异种移植瘤在17个月内连续传代26次。建立了肿瘤株HCC - 94V，接种成功率高（92.9%）且生长稳定。保留了其原发肿瘤的形态和生物学特性。染色体分析显示人类非整倍体模式，数量为9 - 120条。免疫细胞化学技术检测的肿瘤标志物显示高表达，致癌产物低表达。聚合酶链反应（PCR）结果与原发肿瘤组织患者相同，人乳头瘤病毒（HPV）16（+），18（-）。从异种移植瘤中建立了新的人细胞系HCC - 94，并通过131次传代维持了16个月。透射电子显微镜显示细胞间存在典型的桥粒，细胞内有许多张力丝。此外，染色体形态显示出人类肿瘤细胞染色体及突变体的特征。细胞的肿瘤标志物和致癌产物表达高于原发肿瘤。细胞的HPV 16和18，采用PCR检测为阴性。该细胞在裸鼠中具有高度致瘤性。移植瘤在形态上与原发肿瘤组织相似且HPV 16阳性。HCC - 94的细胞克隆培养和无血清培养均成功。
HCC - 94V和HCC - 94可被视为一种新的异种移植瘤株，可能为进一步研究人宫颈癌提供理想的材料和模型。
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