Liu B, Chen H, Wang D
Department of General Surgery, Daping Hospital, Chongqing.
Zhonghua Yi Xue Za Zhi. 1997 Mar;77(3):171-4.
To study the distribution and significance of endothelin-1 (ET-1) and endothelin receptor A (ETAR) mRNA in liver after endotoxemia using situ hybridization.
30 rats were randomized into control and endotoxin groups. The endotoxin group was injected with endotoxin at a dose of 10 mg/kg body wt. Hybridization of ET-1 and ETAR mRNA of the hepatic tissue was proceeded at 3, 6, 12 and 24 hours after endotoxin administration.
By in situ hybridization, the cell expression of ET-1 mRNA was hepatic sinusoids, the endothelial cells of portal vein, and Kupffer cell and the cell expression of ETAR mRNA was the small artery smooth muscle cells of hepatic lobule and the hepatic sinusoidal lining cells. The accumulation of grain was observed in many cells of the liver. The number of accumulating grain increased in unit scope.
ET-1 is synthesized by the hepatic sinusoids, hepatic vascular endothelial cells, and Kupffer cells, ETAR may be located on the hepatic vascular smooth muscle cells and Ito cells (fat-storing cells) around sinusoid. Endotoxin may, on transcription and translation level, lead to the increase of ET-1 and ETAR in synthesis and release.
采用原位杂交技术研究内毒素血症后肝脏中内皮素-1(ET-1)和内皮素A受体(ETAR)mRNA的分布及意义。
将30只大鼠随机分为对照组和内毒素组。内毒素组按10mg/kg体重的剂量注射内毒素。在内毒素给药后3、6、12和24小时对肝组织进行ET-1和ETAR mRNA的杂交。
通过原位杂交,ET-1 mRNA的细胞表达位于肝血窦、门静脉内皮细胞和库普弗细胞,ETAR mRNA的细胞表达位于肝小叶小动脉平滑肌细胞和肝血窦衬里细胞。在肝脏的许多细胞中观察到颗粒聚集。单位视野内聚集颗粒的数量增加。
ET-1由肝血窦、肝血管内皮细胞和库普弗细胞合成,ETAR可能位于肝血管平滑肌细胞和肝血窦周围的贮脂细胞(伊托细胞)上。内毒素可能在转录和翻译水平上导致ET-1和ETAR合成与释放增加。